DNA Structure and Replication problems
- 1. Which of the following is used to determine the appropriate stringency (Wallace rule)?
Options- A. Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
- B. Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
- C. Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
- D. Tm = 69.3° + 0.41 (%)G+C -650/1 Discuss
Correct Answer: Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
- 2. Automated DNA sequencing uses the chain termination method
Options- A. but with an oligonucleotide primer labeled with a fluorescent dye
- B. but with a polynucleotide primer labeled with a fluorescent dye
- C. with no primer
- D. either (b) or (c) Discuss
Correct Answer: but with an oligonucleotide primer labeled with a fluorescent dye
- 3. Number of times the two DNA strands are interwined in circular DNA and is known as
Options- A. helix frequency
- B. linking number
- C. twisting number
- D. none of these Discuss
Correct Answer: linking number
- 4. Nucleosome is
Options- A. an octamer of four histones with H1 protein
- B. an hexamer of three histones with H1 protein
- C. an hexamer of three histones without H1 protein
- D. an octamer of four histones without H1 protein Discuss
Correct Answer: an octamer of four histones without H1 protein
- 5. DNA polymerases I also has
Options- A. 5'-3' exonuclease activity
- B. 3'-5'exonuclease activity
- C. both (a) and (b)
- D. 5'-3' endonuclease activity Discuss
Correct Answer: 3'-5'exonuclease activity
- 6. After one replication in 15N, how many bands should be observed in a cesium chloride gradient?
Options- A. One
- B. Two
- C. Three
- D. A smear Discuss
Correct Answer: One
- 7. Which of the following is correct?
Options- A. Replication starts at a single origin
- B. Replication is bidirectional
- C. Replication is semi conservative
- D. All of the above Discuss
Correct Answer: All of the above
- 8. How does ethidium bromide interact with DNA?
Options- A. It binds to adenine
- B. It binds to phosphate
- C. It intercalates between bases
- D. It does not interact Discuss
Correct Answer: It intercalates between bases
- 9. Replication in E. coli is initiated by the generation of short RNA primers using
Options- A. RNA polymerase
- B. primase
- C. reverse transcriptase
- D. both (a) and (b) Discuss
Correct Answer: both (a) and (b)
- 10. In a typical electrophoresis experiment, larger fragments of DNA move more
Options- A. slowly, so they appear nearer the top of a gel photograph
- B. quickly, so they appear nearer the top of a gel photograph
- C. slowly, so they appear nearer the bottom of a gel photograph
- D. quickly, so they appear nearer the bottom of a gel photograph Discuss
Correct Answer: slowly, so they appear nearer the top of a gel photograph
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