HPLC problems
- 1. Which of the following statements is true for a refractive index detector in HPLC?
Options- A. It is more sensitive than a UV detector
- B. It can only be used for isocratic elutions
- C. It does not respond to many solutes
- D. none of above Discuss
Correct Answer: It does not respond to many solutes
- 2. Which can be used as a mobile phase in HPLC applications?
Options- A. Any compound with solubility in liquid
- B. Any compound with limited solubility in liquid
- C. Any compound with non-solubility in liquid
- D. Any of the above Discuss
Correct Answer: Any compound with solubility in liquid
- 3. Void volume refers to the
Options- A. total volume of eluent in the column the remainder being taken up by the packing material
- B. the volume of solvent contained in a liquid chromatographic column
- C. the time required for the gradient to reach the column
- D. the volume of the column between the point at which solvents are mixed and the beginning of the column Discuss
Correct Answer: total volume of eluent in the column the remainder being taken up by the packing material
- 4. HPLC stands for
Options- A. High Pressure Liquid Chromatography
- B. High Performance Liquid Chromatography
- C. both (a) and (b)
- D. Highly Placed Liquid Chromatography Discuss
Correct Answer: both (a) and (b)
- 5. An eluotropic series
Options- A. ranks solvents by their relative abilities to displace solutes from a given absorbent
- B. ranks column packing material by their relative abilities to retain solutes on the column
- C. is a measure of the solvent adsorption energy
- D. none of the above Discuss
Correct Answer: ranks solvents by their relative abilities to displace solutes from a given absorbent
- 6. HPLC methods include
Options- A. liquid/liquid (partition) chromatography
- B. liquid/solid (adsorption) chromatography
- C. ion exchange and size exclusion chromatography
- D. all of the above Discuss
Correct Answer: all of the above
- 7. In reversed phase HPLC, there is a
Options- A. non polar solvent/polar column
- B. polar solvent/non-polar column
- C. non polar solvent/non-polar column
- D. any of the above Discuss
Correct Answer: polar solvent/non-polar column
- 8. A gradient elution in HPLC is one in which the composition of the solvent
Options- A. remains constant
- B. is changed continuously or in a series of steps
- C. both (a) and (b)
- D. none of the above Discuss
Correct Answer: is changed continuously or in a series of steps
- 9. The eluent strength is a measure of
Options- A. solvent adsorption energy
- B. solvent absorption energy
- C. solvent diffusivity
- D. solvent mixing index Discuss
Correct Answer: solvent adsorption energy
- 10. Dwell volume is defined as
Options- A. the volume of solvent contained in a liquid chromatographic column
- B. the time required for the gradient to reach the column
- C. the volume of the column between the point at which solvents are mixed and the beginning of the column
- D. none of these Discuss
Correct Answer: the volume of the column between the point at which solvents are mixed and the beginning of the column
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