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HPLC problems


  • 1. Which of the following statements is true for a refractive index detector in HPLC?

  • Options
  • A. It is more sensitive than a UV detector
  • B. It can only be used for isocratic elutions
  • C. It does not respond to many solutes
  • D. none of above
  • Discuss
  • 2. Which can be used as a mobile phase in HPLC applications?

  • Options
  • A. Any compound with solubility in liquid
  • B. Any compound with limited solubility in liquid
  • C. Any compound with non-solubility in liquid
  • D. Any of the above
  • Discuss
  • 3. Void volume refers to the

  • Options
  • A. total volume of eluent in the column the remainder being taken up by the packing material
  • B. the volume of solvent contained in a liquid chromatographic column
  • C. the time required for the gradient to reach the column
  • D. the volume of the column between the point at which solvents are mixed and the beginning of the column
  • Discuss
  • 4. HPLC stands for

  • Options
  • A. High Pressure Liquid Chromatography
  • B. High Performance Liquid Chromatography
  • C. both (a) and (b)
  • D. Highly Placed Liquid Chromatography
  • Discuss
  • 5. An eluotropic series

  • Options
  • A. ranks solvents by their relative abilities to displace solutes from a given absorbent
  • B. ranks column packing material by their relative abilities to retain solutes on the column
  • C. is a measure of the solvent adsorption energy
  • D. none of the above
  • Discuss
  • 6. HPLC methods include

  • Options
  • A. liquid/liquid (partition) chromatography
  • B. liquid/solid (adsorption) chromatography
  • C. ion exchange and size exclusion chromatography
  • D. all of the above
  • Discuss
  • 7. In reversed phase HPLC, there is a

  • Options
  • A. non polar solvent/polar column
  • B. polar solvent/non-polar column
  • C. non polar solvent/non-polar column
  • D. any of the above
  • Discuss
  • 8. A gradient elution in HPLC is one in which the composition of the solvent

  • Options
  • A. remains constant
  • B. is changed continuously or in a series of steps
  • C. both (a) and (b)
  • D. none of the above
  • Discuss
  • 9. The eluent strength is a measure of

  • Options
  • A. solvent adsorption energy
  • B. solvent absorption energy
  • C. solvent diffusivity
  • D. solvent mixing index
  • Discuss
  • 10. Dwell volume is defined as

  • Options
  • A. the volume of solvent contained in a liquid chromatographic column
  • B. the time required for the gradient to reach the column
  • C. the volume of the column between the point at which solvents are mixed and the beginning of the column
  • D. none of these
  • Discuss

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