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DNA Structure and Replication problems

  • 1. One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

  • Options
  • A. Conservative theory
  • B. Dispersive theory
  • C. Semi-conservative theory
  • D. Evolutionary theory
    • Discuss
  • 2. The melting of 40-45 bp at the oriC site of E. coli requires

  • Options
  • A. DNA + ATP + HU
  • B. DNA + ATP + HU + DNAb
  • C. DNA+ ATP + HU + DNAb + DNAa
  • D. DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
    • Discuss
  • 3. In DNA replication, one new strand is synthesized complementary to an old (parent) strand and is referred as

  • Options
  • A. mutational
  • B. semiconservative
  • C. discontinuous
  • D. antiparallel
    • Discuss
  • 4. The role of primase is to

  • Options
  • A. dismantle RNA primer
  • B. cleave and unwinds short sections of DNA ahead of the replication fork
  • C. proofread base pairing
  • D. synthesize an RNA primer to begin the elongation process
    • Discuss
  • 5. The replication of a linear DNA molecule in a eukaryotic chromosome creates a problem which is reported to be sorted out by

  • Options
  • A. using enzyme called telomerase
  • B. shortening of the double stranded replicated portion
  • C. using RNA as a template
  • D. none of the above
    • Discuss
  • 6. Which of the following species lack a 'classical' histone H1?

  • Options
  • A. Saccharomyces cerevisiae
  • B. Strongylocentrotus purpuratus
  • C. Xenopus laevis
  • D. Arabidopsis thaliana
    • Discuss
  • 7. The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is

  • Options
  • A. DNA polymerase
  • B. strandase
  • C. helicase
  • D. none of these
    • Discuss
  • 8. The packing ratio of nucleosomes is about

  • Options
  • A. 6
  • B. 8
  • C. 7
  • D. 9
    • Discuss
  • 9. Each replication bubbles consists of

  • Options
  • A. 3 replication forks
  • B. 2 replication forks
  • C. 4 replication forks
  • D. 1 replication forks
    • Discuss
  • 10. The nicks or gaps between Okazaki fragments are eventually sealed by

  • Options
  • A. gyrase
  • B. DNA ligase
  • C. RNA ligase
  • D. polymerase
    • Discuss

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