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Polymerase Chain Reaction (Biochemistry) Question and Answers-Page first

Polymerase Chain Reaction problems


  • 1. What technique can be used to determine the murderer who left blood with the victim?

  • Options
  • A. DNA sequencing
  • B. PCR amplification
  • C. Western blot
  • D. RFLP mapping
  • Discuss
  • 2. PCR can be used to amplify a specific fragment of DNA from which of the following?

  • Options
  • A. A drop of blood
  • B. A hair follicle
  • C. A fragment of skin
  • D. All of these
  • Discuss
  • 3. In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

  • Options
  • A. 16 single strands of DNA
  • B. 16 double stranded DNA
  • C. 18 single strands of DNA
  • D. 18 double stranded DNA
  • Discuss
  • 4. To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

  • Options
  • A. with the same restriction enzyme and mixed together
  • B. with different restriction enzyme and mixed together
  • C. with the combination of enzymes and then seperated
  • D. with the combination of enzymes and mixed together
  • Discuss
  • 5. From a single molecule of DNA, PCR can make

  • Options
  • A. one additional copy
  • B. hundreds of copies
  • C. thousands of copies
  • D. millions of copies
  • Discuss
  • 6. PCR (Polymerase Chain Reaction) can make multiple copies of

  • Options
  • A. DNA
  • B. RNA
  • C. protein
  • D. carbohydrate
  • Discuss
  • 7. Bactriophages absorb to a bacterial surface and inject the phage DNA through the

  • Options
  • A. cell wall into cytosol
  • B. cell wall into plasmamembrane
  • C. both (a) and (b)
  • D. cell wall into endoplasmic reticulum
  • Discuss
  • 8. Additional mono saccharides are added in Golgi to produce

  • Options
  • A. either high mannose type oligosaccharide or a complex type
  • B. either fructose type monosaccharide or a complex type
  • C. either high sucrose type polysaccharide or a complex type
  • D. none of the above
  • Discuss
  • 9. Southern blotting involves

  • Options
  • A. electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
  • B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
  • C. electrophoresis of DNA molecules followed by incubation
  • D. electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
  • Discuss
  • 10. PCR is used

  • Options
  • A. to diagnose genetic diseases
  • B. to solve crimes
  • C. to study gene function
  • D. all of these
  • Discuss

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