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Polymerase Chain Reaction problems
1. What technique can be used to determine the murderer who left blood with the victim?
Options
A. DNA sequencing
B. PCR amplification
C. Western blot
D. RFLP mapping
Show Answer
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Correct Answer: PCR amplification
2. PCR can be used to amplify a specific fragment of DNA from which of the following?
Options
A. A drop of blood
B. A hair follicle
C. A fragment of skin
D. All of these
Show Answer
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Correct Answer: All of these
3. In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to
Options
A. 16 single strands of DNA
B. 16 double stranded DNA
C. 18 single strands of DNA
D. 18 double stranded DNA
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Correct Answer: 16 single strands of DNA
4. To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
Options
A. with the same restriction enzyme and mixed together
B. with different restriction enzyme and mixed together
C. with the combination of enzymes and then seperated
D. with the combination of enzymes and mixed together
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Correct Answer: with the same restriction enzyme and mixed together
5. From a single molecule of DNA, PCR can make
Options
A. one additional copy
B. hundreds of copies
C. thousands of copies
D. millions of copies
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Correct Answer: millions of copies
6. PCR (Polymerase Chain Reaction) can make multiple copies of
Options
A. DNA
B. RNA
C. protein
D. carbohydrate
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Correct Answer: DNA
7. Bactriophages absorb to a bacterial surface and inject the phage DNA through the
Options
A. cell wall into cytosol
B. cell wall into plasmamembrane
C. both (a) and (b)
D. cell wall into endoplasmic reticulum
Show Answer
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Correct Answer: cell wall into cytosol
8. Additional mono saccharides are added in Golgi to produce
Options
A. either high mannose type oligosaccharide or a complex type
B. either fructose type monosaccharide or a complex type
C. either high sucrose type polysaccharide or a complex type
D. none of the above
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Correct Answer: either high mannose type oligosaccharide or a complex type
9. Southern blotting involves
Options
A. electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
C. electrophoresis of DNA molecules followed by incubation
D. electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
Show Answer
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Correct Answer: electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
10. PCR is used
Options
A. to diagnose genetic diseases
B. to solve crimes
C. to study gene function
D. all of these
Show Answer
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Correct Answer: all of these
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More in Biochemistry:
Allosteric Effects
Amino Acid Metabolism
Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
Gel Electrophoresis
Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Immunological Techniques
Lipid
Membrane Structure and Functions
Minerals
Nitrogen Metabolism
NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
Structure and Properties of Peptides
TCA Cycle
Thermodynamics and Free Energy
Transcription and Regulation
UV Luminance Spectroscopy
Vitamins and Coenzymes
Water, pH and Macromolecules