Immunological Techniques problems
- 1. What radioactive molecule is used to end-label an oligonucleotide?
Options- A. (alpha)-32P-ATP
- B. (alpha)-32P-dUTP
- C. (gamma)-32P-ATP
- D. Any of these Discuss
Correct Answer: (gamma)-32P-ATP
- 2. The competitive immunoassay can be used
Options- A. to detect very small amounts of antigen
- B. to detect antibody associated with allergies (IgE)
- C. both (a) and (b)
- D. commonly to detect trace amounts of drugs. Discuss
Correct Answer: both (a) and (b)
- 3. Rocket Immunodiffusion is also known as
Options- A. gel diffusion
- B. electroimmunodiffusion
- C. double-diffusion
- D. none of these Discuss
Correct Answer: electroimmunodiffusion
- 4. Which of the following is a positively charged matrix for ion exchange chromatography?
Options- A. CM cellulose
- B. DEAE cellulose
- C. Phosphocellulose
- D. None of these Discuss
Correct Answer: DEAE cellulose
- 5. What is approximatively the half life of 32-Phosphorus?
Options- A. 14 days
- B. 28 days
- C. 42 days
- D. 30 days Discuss
Correct Answer: 14 days
- 6. What would happen if serum is omitted from the ELISA, but all other steps remain same?
Options- A. Anti-human Ig-conjugate would not bind and be washed away
- B. The OD values would be nearly the same as the assay control
- C. Both (a) and (b)
- D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Discuss
Correct Answer: Both (a) and (b)
- 7. RAST test (radio allergosorbent test) is often used to detect
Options- A. antibodies associated with allergies (IgE)
- B. antigen associated with allergies (IgE)
- C. bacteriophages
- D. None of the above Discuss
Correct Answer: antibodies associated with allergies (IgE)
- 8. What is the ELISA test intended to measure?
Options- A. Antibody to HIV only
- B. Antigen to HIV only
- C. Presence of free, circulating virus in the patient
- D. Antibodies directed against HLA molecules Discuss
Correct Answer: Antibody to HIV only
- 9. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?
Options- A. Open circular, super coiled, linear plasmid DNA
- B. Linear plasmid, open circular, super coiled plasmid
- C. Open circular, super coiled, linear plasmid DNA
- D. Open circular, linear plasmid, super coiled plasmid Discuss
Correct Answer: Linear plasmid, open circular, super coiled plasmid
- 10. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
Options- A. 2000 kb and 20kb DNA
- B. 1000 kb and l0kb DNA
- C. 20 kb and 2000 kb DNA
- D. 10 kb and 1000 kb DNA Discuss
Correct Answer: 20 kb and 2000 kb DNA
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