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Immunological Techniques (Biochemistry) Question and Answers-Page first

Immunological Techniques problems


  • 1. What radioactive molecule is used to end-label an oligonucleotide?

  • Options
  • A. (alpha)-32P-ATP
  • B. (alpha)-32P-dUTP
  • C. (gamma)-32P-ATP
  • D. Any of these
  • Discuss
  • 2. The competitive immunoassay can be used

  • Options
  • A. to detect very small amounts of antigen
  • B. to detect antibody associated with allergies (IgE)
  • C. both (a) and (b)
  • D. commonly to detect trace amounts of drugs.
  • Discuss
  • 3. Rocket Immunodiffusion is also known as

  • Options
  • A. gel diffusion
  • B. electroimmunodiffusion
  • C. double-diffusion
  • D. none of these
  • Discuss
  • 4. Which of the following is a positively charged matrix for ion exchange chromatography?

  • Options
  • A. CM cellulose
  • B. DEAE cellulose
  • C. Phosphocellulose
  • D. None of these
  • Discuss
  • 5. What is approximatively the half life of 32-Phosphorus?

  • Options
  • A. 14 days
  • B. 28 days
  • C. 42 days
  • D. 30 days
  • Discuss
  • 6. What would happen if serum is omitted from the ELISA, but all other steps remain same?

  • Options
  • A. Anti-human Ig-conjugate would not bind and be washed away
  • B. The OD values would be nearly the same as the assay control
  • C. Both (a) and (b)
  • D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
  • Discuss
  • 7. RAST test (radio allergosorbent test) is often used to detect

  • Options
  • A. antibodies associated with allergies (IgE)
  • B. antigen associated with allergies (IgE)
  • C. bacteriophages
  • D. None of the above
  • Discuss
  • 8. What is the ELISA test intended to measure?

  • Options
  • A. Antibody to HIV only
  • B. Antigen to HIV only
  • C. Presence of free, circulating virus in the patient
  • D. Antibodies directed against HLA molecules
  • Discuss
  • 9. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

  • Options
  • A. Open circular, super coiled, linear plasmid DNA
  • B. Linear plasmid, open circular, super coiled plasmid
  • C. Open circular, super coiled, linear plasmid DNA
  • D. Open circular, linear plasmid, super coiled plasmid
  • Discuss
  • 10. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

  • Options
  • A. 2000 kb and 20kb DNA
  • B. 1000 kb and l0kb DNA
  • C. 20 kb and 2000 kb DNA
  • D. 10 kb and 1000 kb DNA
  • Discuss

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