Protein Purification problems
- 1. In ion-exchange chromatography
Options- A. proteins are separated on the basis of their net charge
- B. proteins are separated on the basis of their size
- C. proteins are separated on the basis of their shape
- D. either (b) or (c) Discuss
Correct Answer: proteins are separated on the basis of their net charge
- 2. Which of the following may be added to stabilize the protein after yeast cells disruption?
Options- A. NaCl
- B. Protease inhibitor
- C. AMP
- D. All of these Discuss
Correct Answer: All of these
- 3. Affinity chromatography deals with the
Options- A. specific binding of a protein constituents for another molecule
- B. protein - protein interaction
- C. protein - carbohydrate interaction
- D. none of the above Discuss
Correct Answer: specific binding of a protein constituents for another molecule
- 4. A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
Options- A. 1,000 units/mg
- B. 10,000 units/mg.
- C. 100,000 units/mg
- D. 1,000,000 units/mg Discuss
Correct Answer: 100,000 units/mg
- 5. During successful purification scheme, this may be expected that the
Options- A. specific activity increases
- B. specific activity decreases
- C. number of proteins in the sample decreases
- D. both (a) and (c) Discuss
Correct Answer: both (a) and (c)
- 6. Proteins separation can be carried out on the basis of
Options- A. net charge
- B. solubility in salt solutions
- C. size or mass
- D. all of these Discuss
Correct Answer: all of these
- 7. Gel-filtration chromatography separates on the basis of
Options- A. size and shape using porous beads packed in a column
- B. size using porous beads packed in a column
- C. shape using porous beads packed in a column
- D. none of the above Discuss
Correct Answer: size and shape using porous beads packed in a column
- 8. The best way to determine the location of protein in the purification scheme is to measure the
Options- A. rate of ATP synthesis
- B. changes in the refractive index
- C. UV absorption
- D. mass spectroscopy of the protein Discuss
Correct Answer: rate of ATP synthesis
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