FT IR Spectroscopy problems
- 1. Cytochrome C has an isoelectric pH of
Options- A. 8.5
- B. 10.05
- C. 7.5
- D. 11.05 Discuss
Correct Answer: 10.05
- 2. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
Options- A. Molecular mass
- B. Solubility
- C. Isoelectric point
- D. Folding Discuss
Correct Answer: Isoelectric point
- 3. How do you turn a signal recorded in the time domain into a frequency domain signal?
Options- A. Fourier transformation
- B. Measurement of peak areas
- C. By use of a Michelson interferometer
- D. None of the above Discuss
Correct Answer: Fourier transformation
- 4. Negative staining is a technique used in
Options- A. electron microscopy
- B. gel electrophoresis
- C. immunocytochemistry
- D. light microscopy Discuss
Correct Answer: electron microscopy
- 5. In scanning electron microscopy
Options- A. a specimen is fixed and then coated with thin layer of a heavy metal
- B. a specimen is fixed and then coated with transition metal
- C. a specimen is not fixed and then coated with thin layer of a heavy metal
- D. none of the above Discuss
Correct Answer: a specimen is fixed and then coated with thin layer of a heavy metal
- 6. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
Options- A. 2
- B. 4
- C. 3
- D. 6 Discuss
Correct Answer: 2
- 7. In FT-NMR, how are nuclei excited?
Options- A. By radio-frequency radiation whose frequency is swept across a predetermined range
- B. By an intense pulse of radiation which contains a wide range of frequencies
- C. By an intense pressure
- D. None of the above Discuss
Correct Answer: By radio-frequency radiation whose frequency is swept across a predetermined range
- 8. In immunofluroscence microscopy, fluroscent compounds are attached to
Options- A. an antibody specific for the subcellular structure
- B. an antigen specific for the subcellular structure
- C. bound antibody specific for the subcellular structure
- D. none of these Discuss
Correct Answer: an antibody specific for the subcellular structure
- 9. What is meant by rotating frame of reference?
Options- A. That the sample is spun rapidly in the applied field
- B. If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
- C. That the detector rotates around the sample
- D. None of the above Discuss
Correct Answer: If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
- 10. The sequence of amino acids in proteins can be determined by means of
Options- A. identification of the -NH2 terminal amino acids
- B. identification of the -COOH terminal amino acids
- C. partial cleavage of the original polypeptide into smaller polypeptides
- D. all of the above Discuss
Correct Answer: all of the above
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