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Chromatography (Biochemistry) Question and Answers-Page first

Chromatography problems


  • 1. In gas chromatography, the basis for separation of the components of the volatile material is the difference in

  • Options
  • A. partition coefficients
  • B. conductivity
  • C. molecular weight
  • D. molarity
  • Discuss
  • 2. Ion exchange chromatography is based on the

  • Options
  • A. electrostatic attraction
  • B. electrical mobility of ionic species
  • C. adsorption chromatography
  • D. partition chromatography
  • Discuss
  • 3. In reverse phase chromatography, the stationary phase is made

  • Options
  • A. non-polar
  • B. polar
  • C. either non-polar or polar
  • D. none of these
  • Discuss
  • 4. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

  • Options
  • A. V = V0 + kDVi
  • B. V = V0/Vi
  • C. V = V0 - kDVi
  • D. V/V0 = kDVi
  • Discuss
  • 5. Thin layer chromatography is

  • Options
  • A. partition chromatography
  • B. electrical mobility of ionic species
  • C. adsorption chromatography
  • D. none of the above
  • Discuss
  • 6. A combination of paper chromatography and electrophoresis involves

  • Options
  • A. partition chromatography
  • B. electrical mobility of the ionic species
  • C. both (a) and (b)
  • D. none of these
  • Discuss

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