Transcription and Regulation problems
- 1. Upstream regions which are essential for correct transcription are called
Options- A. enhancers
- B. start codons
- C. promoters
- D. transcription factors Discuss
Correct Answer: promoters
- 2. Immediately after transcription
Options- A. a methylated guanine cap is added to the 51 end of the transcript
- B. a methylated guanine cap is added to the 31 end of the transcript
- C. both (a) and (b)
- D. a poly adenylation signal is added Discuss
Correct Answer: a methylated guanine cap is added to the 51 end of the transcript
- 3. RNA polymerase in prokaryotes has a removable
Options- A. alpha subunit
- B. beta subunit
- C. both (a) and (b)
- D. sigma subunit Discuss
Correct Answer: sigma subunit
- 4. Transcription factors are
Options- A. promoters
- B. proteins which bind to DNA and regulate transcription
- C. TATA boxes
- D. CATT boxes Discuss
Correct Answer: proteins which bind to DNA and regulate transcription
- 5. Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?
Options- A. Presence of glucose in the growth media
- B. Presence of lactose in the growth media
- C. Presence of IPTG (isopropyl thiogalactoside) in the growth media
- D. Both (b) and (c) Discuss
Correct Answer: Both (b) and (c)
- 6. Rho-dependent termination of transcription in E. coli
Options- A. requires ATP
- B. requires about 50 nucleotides of uncomplexed mRNA
- C. both (a) and (b)
- D. removes mRNA and holoenzyme from the DNA Discuss
Correct Answer: both (a) and (b)
- 7. The transcriptional regulatory proteins in eukaryotes which induce transcription usually have
Options- A. at least two distinct domains of protein structure, a DNA binding domain and an activation domain
- B. at least two distinct domains of protein structure and a DNA binding domain
- C. at least one distinct domains of protein structure, a DNA binding domain and an activation domain
- D. none of these Discuss
Correct Answer: at least two distinct domains of protein structure, a DNA binding domain and an activation domain
- 8. The closed complex at bacterial promoters is
Options- A. in equilibrium with free RNA polymerase and the promoter
- B. not affected by promoter mutations
- C. bound more tightly when repressors are present
- D. not affected by activator proteins Discuss
Correct Answer: in equilibrium with free RNA polymerase and the promoter
- 9. The complex of RNA polymerase, DNA template and new RNA transcript is called
Options- A. transcription bubble
- B. replication bubble
- C. a translation bubble
- D. none of these Discuss
Correct Answer: transcription bubble
- 10. The binding of lac repressor to DNA could be considered to be analogous to
Options- A. competitive inhibition of an enzyme
- B. mixed-type inhibition of an enzyme
- C. uncompetitive inhibition of an enzyme
- D. allosteric effects in enzyme regulation Discuss
Correct Answer: competitive inhibition of an enzyme
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