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Transcription and Regulation (Biochemistry) Question and Answers-Page first

Transcription and Regulation problems


  • 1. Upstream regions which are essential for correct transcription are called

  • Options
  • A. enhancers
  • B. start codons
  • C. promoters
  • D. transcription factors
  • Discuss
  • 2. Immediately after transcription

  • Options
  • A. a methylated guanine cap is added to the 51 end of the transcript
  • B. a methylated guanine cap is added to the 31 end of the transcript
  • C. both (a) and (b)
  • D. a poly adenylation signal is added
  • Discuss
  • 3. RNA polymerase in prokaryotes has a removable

  • Options
  • A. alpha subunit
  • B. beta subunit
  • C. both (a) and (b)
  • D. sigma subunit
  • Discuss
  • 4. Transcription factors are

  • Options
  • A. promoters
  • B. proteins which bind to DNA and regulate transcription
  • C. TATA boxes
  • D. CATT boxes
  • Discuss
  • 5. Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?

  • Options
  • A. Presence of glucose in the growth media
  • B. Presence of lactose in the growth media
  • C. Presence of IPTG (isopropyl thiogalactoside) in the growth media
  • D. Both (b) and (c)
  • Discuss
  • 6. Rho-dependent termination of transcription in E. coli

  • Options
  • A. requires ATP
  • B. requires about 50 nucleotides of uncomplexed mRNA
  • C. both (a) and (b)
  • D. removes mRNA and holoenzyme from the DNA
  • Discuss
  • 7. The transcriptional regulatory proteins in eukaryotes which induce transcription usually have

  • Options
  • A. at least two distinct domains of protein structure, a DNA binding domain and an activation domain
  • B. at least two distinct domains of protein structure and a DNA binding domain
  • C. at least one distinct domains of protein structure, a DNA binding domain and an activation domain
  • D. none of these
  • Discuss
  • 8. The closed complex at bacterial promoters is

  • Options
  • A. in equilibrium with free RNA polymerase and the promoter
  • B. not affected by promoter mutations
  • C. bound more tightly when repressors are present
  • D. not affected by activator proteins
  • Discuss
  • 9. The complex of RNA polymerase, DNA template and new RNA transcript is called

  • Options
  • A. transcription bubble
  • B. replication bubble
  • C. a translation bubble
  • D. none of these
  • Discuss
  • 10. The binding of lac repressor to DNA could be considered to be analogous to

  • Options
  • A. competitive inhibition of an enzyme
  • B. mixed-type inhibition of an enzyme
  • C. uncompetitive inhibition of an enzyme
  • D. allosteric effects in enzyme regulation
  • Discuss

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