Gel Electrophoresis problems
- 1. In a native PAGE, proteins are separated on the basis of
Options- A. net negative charge
- B. net charge and size
- C. net positive charges size
- D. net positive charge Discuss
Correct Answer: net charge and size
- 2. In SDS-PAGE, the protein sample is first
Options- A. treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
- B. fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.
- C. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
- D. none of the above Discuss
Correct Answer: treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
- 3. Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in
Options- A. both proteins migrate to the anode
- B. histones migrate to the anode and myoglobin migrates to the cathode
- C. histones migrate to the cathode and myoglobin migrates to the anode
- D. both proteins migrate to the cathode Discuss
Correct Answer: histones migrate to the cathode and myoglobin migrates to the anode
- 4. Proteins are separated in an SDS-PAGE experiment on the basis of their
Options- A. positively charged side chains
- B. molecular weight
- C. negatively charged side chains
- D. different isoelectric points Discuss
Correct Answer: molecular weight
- 5. In an SDS-PAGE
Options- A. proteins are denatured by the SDS
- B. proteins have the same charge-to-mass ratio
- C. smaller proteins migrate more rapidly through the gel
- D. all of the above Discuss
Correct Answer: all of the above
- 6. The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by
Options- A. SDS-PAGE electrophoresis
- B. gel filtration chromatography
- C. combining information from (a)and (b)
- D. isoelectric focusing Discuss
Correct Answer: combining information from (a)and (b)
- 7. In isoelectric focusing, proteins are separated on the basis of their
Options- A. relative content of positively charged residue only
- B. relative content of negatively charged residue only
- C. size
- D. relative content of positively and negatively charged residue Discuss
Correct Answer: relative content of positively and negatively charged residue
- 8. In a gel filtration column
Options- A. smaller proteins enter the beads more readily
- B. large proteins elute first
- C. both (a) and (b)
- D. large proteins enter the beads more readily Discuss
Correct Answer: both (a) and (b)
- 9. Proteins can be visualized directly in gels by
Options- A. staining them with the dye
- B. using electron microscope only
- C. measuring their molecular weight
- D. none of these Discuss
Correct Answer: staining them with the dye
More in Biochemistry:
Programming
Copyright ©CuriousTab. All rights reserved.