Protein and Nucleic Acid Interactions problems
- 1. Reverse transcripase from HIV does not
Options- A. uses RNA as a template
- B. has a 5' to 3' polymerase activity
- C. has a 3'- 5' exonuclease activity
- D. requires a primer Discuss
Correct Answer: has a 3'- 5' exonuclease activity
- 2. Cleavage of DNA by EcoRI endonuclease results in the formation of
Options- A. two 3'-OH ends and two 5'-phosphate ends
- B. one 3'-OH end and one 5'-phosphate end
- C. two 3'-phosphate ends and two 3'-OH ends
- D. one 3'-OH end and two 5'-phosphate end Discuss
Correct Answer: two 3'-OH ends and two 5'-phosphate ends
- 3. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
Options- A. Ile and Val
- B. Lys and Arg
- C. Leu and Ala
- D. Cys and Met Discuss
Correct Answer: Lys and Arg
- 4. The type II restriction endonucleases, bind to DNA sites
Options- A. using H-bonds for specificity
- B. that are usually palindromic
- C. using non-sequence-dependent backbone interactions
- D. all of the above Discuss
Correct Answer: all of the above
- 5. The enzyme that joins DNA cuts is called
Options- A. joinase
- B. ligase
- C. DNA phosphorylase
- D. reverse transcriptase Discuss
Correct Answer: ligase
- 6. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
Options- A. positive direction
- B. 3' to 5' direction
- C. 5' to 3' direction
- D. negative direction Discuss
Correct Answer: 5' to 3' direction
- 7. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
Options- A. Asn and Gln
- B. Cys and Met
- C. Lys and Leu
- D. Ile and Val Discuss
Correct Answer: Asn and Gln
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