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Protein and Nucleic Acid Interactions problems


  • 1. Reverse transcripase from HIV does not

  • Options
  • A. uses RNA as a template
  • B. has a 5' to 3' polymerase activity
  • C. has a 3'- 5' exonuclease activity
  • D. requires a primer
  • Discuss
  • 2. Cleavage of DNA by EcoRI endonuclease results in the formation of

  • Options
  • A. two 3'-OH ends and two 5'-phosphate ends
  • B. one 3'-OH end and one 5'-phosphate end
  • C. two 3'-phosphate ends and two 3'-OH ends
  • D. one 3'-OH end and two 5'-phosphate end
  • Discuss
  • 3. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

  • Options
  • A. Ile and Val
  • B. Lys and Arg
  • C. Leu and Ala
  • D. Cys and Met
  • Discuss
  • 4. The type II restriction endonucleases, bind to DNA sites

  • Options
  • A. using H-bonds for specificity
  • B. that are usually palindromic
  • C. using non-sequence-dependent backbone interactions
  • D. all of the above
  • Discuss
  • 5. The enzyme that joins DNA cuts is called

  • Options
  • A. joinase
  • B. ligase
  • C. DNA phosphorylase
  • D. reverse transcriptase
  • Discuss
  • 6. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

  • Options
  • A. positive direction
  • B. 3' to 5' direction
  • C. 5' to 3' direction
  • D. negative direction
  • Discuss
  • 7. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

  • Options
  • A. Asn and Gln
  • B. Cys and Met
  • C. Lys and Leu
  • D. Ile and Val
  • Discuss

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