Nucleic Acids problems
- 1. The glycosidic bonds in DNA and RNA
Options- A. connect the sugar to the base
- B. can be hydrolyzed by OH- ion
- C. stabilize Watson-Crick H-bonds
- D. are free to rotate over about 180° Discuss
Correct Answer: connect the sugar to the base
- 2. Thymidine
Options- A. can participate in hydrophobic interactions due to its methyl group
- B. is replaced by uracil in RNA
- C. normally forms two hydrogen bonds with adenosine
- D. all of the above Discuss
Correct Answer: all of the above
- 3. In gel electrophoresis, what fragments will move most quickly through a gel?
Options- A. Large fragments
- B. Small fragments
- C. Large genome
- D. None of these Discuss
Correct Answer: Small fragments
- 4. A purine with an amine (NH2) group on the 6th carbon is
Options- A. adenine
- B. cytosine
- C. thymine
- D. guanine Discuss
Correct Answer: adenine
- 5. Because DNA is a highly charged polyanion, its stability to heat denaturation
Options- A. does not depend on hydrophobic interactions
- B. increases with increasing salt
- C. is independent of G - C content
- D. decreases with increasing salt Discuss
Correct Answer: increases with increasing salt
- 6. The unfavourable free energy associated with not satisfying a hydrogen bond after formation of the double helix is approximately
Options- A. 10 kJ/mol
- B. 20 kJ/mol
- C. 25 kJ/mol
- D. 30 kJ/mol Discuss
Correct Answer: 20 kJ/mol
- 7. Nucleotide bases and aromatic amino acids absorb light respectively at
Options- A. 280 and 260 nm
- B. 260 and 280 nm
- C. 270 and 280 nm
- D. 260 and 270 nm Discuss
Correct Answer: 260 and 280 nm
- 8. A nucleotide consists of
Options- A. a sugar, a base and a phosphate
- B. a sugar and a phosphate
- C. paired bases
- D. a sugar, a base and three phosphates Discuss
Correct Answer: a sugar, a base and a phosphate
- 9. Which of the following is a purine?
Options- A. Cytosine
- B. Adenine
- C. Thymine
- D. Uracil Discuss
Correct Answer: Adenine
- 10. A five carbon sugar lacking a hydrogen at the number 2 carbon is found in
Options- A. DNA
- B. RNA
- C. mRNA
- D. rDNA Discuss
Correct Answer: DNA
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