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Gas Chromatography (Biochemistry) Question and Answers-Page first

Gas Chromatography problems


  • 1. Which of the following gases is unsuitable for use as a GC carrier gas?

  • Options
  • A. Nitrogen
  • B. Helium
  • C. Oxygen
  • D. All of the above
  • Discuss
  • 2. Which of the following are not used as stationary phases in a GC column?

  • Options
  • A. Polysiloxanes
  • B. Silica
  • C. Cyclodextrins
  • D. None are used as stationary phases
  • Discuss
  • 3. Which of the following detectors give concentration-dependent signals?

  • Options
  • A. Electron-capture detector
  • B. Thermal conductivity
  • C. Infra-red detector
  • D. All of these
  • Discuss
  • 4. Headspace analysis is carried out in order to

  • Options
  • A. analyse volatile compounds from solid or liquid samples
  • B. determine the psychological state of the tutor
  • C. analyse the column contents ahead of the sample
  • D. determine non-volatiles
  • Discuss
  • 5. Theoretical plates are used to

  • Options
  • A. estimate the efficiency of a column
  • B. determine the thickness of the stationary phase
  • C. measure the distribution of the analyte between mobile and stationary phases
  • D. None of the above
  • Discuss
  • 6. What is the typical internal diameter of fused silica capillary columns?

  • Options
  • A. 0.2-0.3 mm
  • B. 0.3-0.5mm
  • C. 0.5-1.0 mm
  • D. 1.0-2.0 mm
  • Discuss
  • 7. A retention gap is placed between the injector and the front of the column to

  • Options
  • A. retain contaminants and prevent them from reaching the column
  • B. retain the sample and release it gradually to the column
  • C. prevent backflush of the injected solution
  • D. all of the above
  • Discuss
  • 8. Split injection is carried out by

  • Options
  • A. splitting the sample into smaller portions to inject sequentially
  • B. splitting the sample into smaller portions to inject at the same time through parallel ports
  • C. splitting off some of the sample so that it does not enter the column
  • D. none of the above
  • Discuss
  • 9. What useful information can be found from a Van Deemter plot?

  • Options
  • A. The selectivity factor
  • B. Optimum mobile phase flow rate
  • C. Optimum column temperature
  • D. Optimum column length
  • Discuss
  • 10. The GC trace obtained after an experiment is called a

  • Options
  • A. chromatograph
  • B. chromatogram
  • C. chromatophore
  • D. graph
  • Discuss

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