Recombinant DNA Technology problems
- 1. Which of these genes codes for a protein that plays a role in white blood cell function?
Options- A. DCP1
- B. MPO
- C. GLUT4
- D. RP13 Discuss
Correct Answer: MPO
- 2. The TP53 gene of chromosome 17 codes for a protein __________ .
Options- A. that plays a role in the digestive process
- B. involved in glucose transport
- C. involved in the regulation of the cell cycle
- D. that is like a white blood cell protein Discuss
Correct Answer: involved in the regulation of the cell cycle
- 3. An example of a restriction fragment length polymorphism is
Options- A. an Eco RI cuts DNA at a different sequence than Hind III
- B. different length fragments of DNA resulting from loss or gain of a restriction site
- C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
- D. all of the above Discuss
Correct Answer: different length fragments of DNA resulting from loss or gain of a restriction site
- 4. An expression vector
Options- A. always contains an origin of replication
- B. usually contains a gene that confers antibiotic resistance to the bacterial host
- C. always contains DNA segments for the regulation of mRNA production
- D. all of the above Discuss
Correct Answer: all of the above
- 5. Which of the following is correct in terms of determination of location of genetic traits?
Options- A. Known protein coding sequences are too far apart to allow linkage determination for most new genes
- B. Restriction sites allow DNAs to be digested
- C. Protein-coding genes are always associated with a restriction pattern
- D. None of the above Discuss
Correct Answer: Known protein coding sequences are too far apart to allow linkage determination for most new genes
- 6. Plant transformation can be obtained by
Options- A. combining plant and animal cells in culture
- B. shooting DNA into plant cells with a gun
- C. using the E. coli bacterium to infect plant roots
- D. infecting plants with a tobacco mosaic virus Discuss
Correct Answer: shooting DNA into plant cells with a gun
- 7. In order to insert a foreign gene into a plasmid, both must __________
Options- A. have identical DNA sequences
- B. originate from the same type of cell
- C. be cut by the same restriction enzyme
- D. be of the same length Discuss
Correct Answer: be cut by the same restriction enzyme
- 8. In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.
Options- A. negative ... positive
- B. basic ... acidic
- C. long ... short
- D. positive to negative Discuss
Correct Answer: negative ... positive
- 9. Which of the following techniques can be used to determine the defective gene and for developing cancer?
Options- A. Western blot
- B. Southern blot
- C. Northern blot
- D. Eastern blot Discuss
Correct Answer: Southern blot
- 10. Isoschizomers recognize
Options- A. same recognition sequence but different recognition site
- B. same recognition site and recognition sequence
- C. same recognition site and different recognition sequence
- D. different recognition site and different recognition sequence Discuss
Correct Answer: same recognition site and recognition sequence
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