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DNA Structure and Replication problems


  • 1. DNA gyrase in E. coli

  • Options
  • A. adds positive supercoils to chromosomal DNA
  • B. can be inhibited with antibiotics
  • C. is required only at the oriC site
  • D. performs the same function as helicase in eukaryotes
  • Discuss
  • 2. Enzyme, responsible for proofreading base pairing is

  • Options
  • A. DNA polymerase
  • B. Telomerase
  • C. Primase
  • D. DNA ligase
  • Discuss
  • 3. Klenow fragment without free nucleotides exhibits

  • Options
  • A. exonuclease activity
  • B. endonuclease activity
  • C. nickase activity
  • D. no activity
  • Discuss
  • 4. In DNA, guanine pairs with

  • Options
  • A. Adenine
  • B. Cytosine
  • C. Thymine
  • D. Uracil
  • Discuss
  • 5. The accepted hypothesis for DNA replication is

  • Options
  • A. conservative theory
  • B. dispersive theory
  • C. semi-conservative theory
  • D. evolutionary theory
  • Discuss
  • 6. What is the approximate size (in kb) of the E. coli genome?

  • Options
  • A. 3000 kilobase
  • B. 4500 kilobase
  • C. 5500 kilobase
  • D. 6500 kilobase
  • Discuss
  • 7. Which of the following would not contain DNA?

  • Options
  • A. Yeast
  • B. Bacteria
  • C. Glass crystals
  • D. Mold
  • Discuss
  • 8. In sperm heads, DNA is particularly highly condensed and the histones are replaced with small basic protein called

  • Options
  • A. protamines
  • B. purines
  • C. pyrimidines
  • D. all of the above
  • Discuss
  • 9. What is the main damaging effect of UV radiation on DNA?

  • Options
  • A. Depurination
  • B. Formation of thymine dimers
  • C. Single strand break
  • D. Dehydration
  • Discuss
  • 10. Which is the largest among the followings?

  • Options
  • A. Nucleotide
  • B. Nitrogenous base
  • C. Phosphate
  • D. Carbon
  • Discuss

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