Polymerase Chain Reaction problems
- 1. Double stranded DNA denaturation with specified limit of temperature is
Options- A. reversible reaction
- B. irreversible reaction
- C. either (a) or (b)
- D. none of these Discuss
Correct Answer: reversible reaction
- 2. Specialized transduction occurs when
Options- A. the bacteriophage incorporates randomly in the bacterial chromosome
- B. the bacteriophage never incorporates into the bacterial chromosome
- C. the bacteriophage always incorporates at the same position in the bacterial chromosome
- D. none of the above Discuss
Correct Answer: the bacteriophage always incorporates at the same position in the bacterial chromosome
- 3. Genomic libraries are made from
Options- A. genomic DNA of an organism
- B. genomic RNA of an organism
- C. genomic cDNA of an organism
- D. genomic mRNA of an organism Discuss
Correct Answer: genomic DNA of an organism
- 4. Which of the following is correct?
Options- A. HIV is a retrovirus that kills human helper T cells
- B. Causes acquired immune deficiency syndrome (AIDS)
- C. Cripples the immunity systems
- D. All of the above Discuss
Correct Answer: All of the above
- 5. Restriction maps
Options- A. allows comparison between DNA molecules without the need to determine nucleotide sequence
- B. allows comparison between DNA molecules but requires to determine nucleotide sequence
- C. does not allow comparison between DNA molecules
- D. none of the above Discuss
Correct Answer: allows comparison between DNA molecules without the need to determine nucleotide sequence
- 6. A PCR cycle consists of
Options- A. three steps, denaturation, primer annealing and elongation
- B. three steps, denaturation, initiation and elongation
- C. three steps, primer annealing, elongation and termination
- D. three steps, initiation, elongation and termination Discuss
Correct Answer: three steps, denaturation, primer annealing and elongation
- 7. The viruses that can achieve neoplastic transformation are called
Options- A. DNA tumor viruses
- B. RNA tumor viruses
- C. retroviruses HIV
- D. none of these Discuss
Correct Answer: DNA tumor viruses
- 8. PCR amplifies millions of copies of
Options- A. a short fragment of DNA
- B. the entire genome (all the DNA in a sample)
- C. both (a) and (b)
- D. short chain RNA Discuss
Correct Answer: a short fragment of DNA
- 9. Transformation means
Options- A. formation of a pilus
- B. acquiring DNA from the bacterial cell environment
- C. plasmid containing a F factor
- D. F+ and F- strains of bacteria Discuss
Correct Answer: acquiring DNA from the bacterial cell environment
- 10. PCR can be used in
Options- A. cloning
- B. sequencing
- C. medical diagnosis and forensic medicine
- D. all of the above Discuss
Correct Answer: all of the above
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