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Home Biochemistry Polymerase Chain Reaction Comments

  • Question
  • In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to


  • Options
  • A. 16 single strands of DNA
  • B. 16 double stranded DNA
  • C. 18 single strands of DNA
  • D. 18 double stranded DNA

  • Correct Answer
  • 16 single strands of DNA 


  • Polymerase Chain Reaction problems


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    • 1. PCR can be used to amplify a specific fragment of DNA from which of the following?

    • Options
    • A. A drop of blood
    • B. A hair follicle
    • C. A fragment of skin
    • D. All of these
    • Discuss
    • 2. What technique can be used to determine the murderer who left blood with the victim?

    • Options
    • A. DNA sequencing
    • B. PCR amplification
    • C. Western blot
    • D. RFLP mapping
    • Discuss
    • 3. In the presence of tryptophan in the cell, the repressor is __________

    • Options
    • A. bound to tryptophan
    • B. bound to DNA
    • C. bound to both DNA and tryptophan
    • D. bound to neither tryptophan nor DNA
    • Discuss
    • 4. The lac operon is translated into __________ proteins.

    • Options
    • A. 1
    • B. 2
    • C. 3
    • D. 4
    • Discuss
    • 5. Sex hormones activate transcription of specific genes by

    • Options
    • A. binding to a transcription factor
    • B. binding to RNA polymerase
    • C. binding to DNA enhancer region
    • D. binding to DNA promoter region
    • Discuss
    • 6. To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

    • Options
    • A. with the same restriction enzyme and mixed together
    • B. with different restriction enzyme and mixed together
    • C. with the combination of enzymes and then seperated
    • D. with the combination of enzymes and mixed together
    • Discuss
    • 7. From a single molecule of DNA, PCR can make

    • Options
    • A. one additional copy
    • B. hundreds of copies
    • C. thousands of copies
    • D. millions of copies
    • Discuss
    • 8. PCR (Polymerase Chain Reaction) can make multiple copies of

    • Options
    • A. DNA
    • B. RNA
    • C. protein
    • D. carbohydrate
    • Discuss
    • 9. Bactriophages absorb to a bacterial surface and inject the phage DNA through the

    • Options
    • A. cell wall into cytosol
    • B. cell wall into plasmamembrane
    • C. both (a) and (b)
    • D. cell wall into endoplasmic reticulum
    • Discuss
    • 10. Additional mono saccharides are added in Golgi to produce

    • Options
    • A. either high mannose type oligosaccharide or a complex type
    • B. either fructose type monosaccharide or a complex type
    • C. either high sucrose type polysaccharide or a complex type
    • D. none of the above
    • Discuss


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