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Home Biochemistry Polymerase Chain Reaction Comments

  • Question
  • PCR is used


  • Options
  • A. to diagnose genetic diseases
  • B. to solve crimes
  • C. to study gene function
  • D. all of these

  • Correct Answer
  • all of these 


  • Polymerase Chain Reaction problems


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    • 1. Southern blotting involves

    • Options
    • A. electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
    • B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
    • C. electrophoresis of DNA molecules followed by incubation
    • D. electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
    • Discuss
    • 2. Additional mono saccharides are added in Golgi to produce

    • Options
    • A. either high mannose type oligosaccharide or a complex type
    • B. either fructose type monosaccharide or a complex type
    • C. either high sucrose type polysaccharide or a complex type
    • D. none of the above
    • Discuss
    • 3. Bactriophages absorb to a bacterial surface and inject the phage DNA through the

    • Options
    • A. cell wall into cytosol
    • B. cell wall into plasmamembrane
    • C. both (a) and (b)
    • D. cell wall into endoplasmic reticulum
    • Discuss
    • 4. PCR (Polymerase Chain Reaction) can make multiple copies of

    • Options
    • A. DNA
    • B. RNA
    • C. protein
    • D. carbohydrate
    • Discuss
    • 5. From a single molecule of DNA, PCR can make

    • Options
    • A. one additional copy
    • B. hundreds of copies
    • C. thousands of copies
    • D. millions of copies
    • Discuss
    • 6. Double stranded DNA denaturation with specified limit of temperature is

    • Options
    • A. reversible reaction
    • B. irreversible reaction
    • C. either (a) or (b)
    • D. none of these
    • Discuss
    • 7. Specialized transduction occurs when

    • Options
    • A. the bacteriophage incorporates randomly in the bacterial chromosome
    • B. the bacteriophage never incorporates into the bacterial chromosome
    • C. the bacteriophage always incorporates at the same position in the bacterial chromosome
    • D. none of the above
    • Discuss
    • 8. Genomic libraries are made from

    • Options
    • A. genomic DNA of an organism
    • B. genomic RNA of an organism
    • C. genomic cDNA of an organism
    • D. genomic mRNA of an organism
    • Discuss
    • 9. Which of the following is correct?

    • Options
    • A. HIV is a retrovirus that kills human helper T cells
    • B. Causes acquired immune deficiency syndrome (AIDS)
    • C. Cripples the immunity systems
    • D. All of the above
    • Discuss
    • 10. Restriction maps

    • Options
    • A. allows comparison between DNA molecules without the need to determine nucleotide sequence
    • B. allows comparison between DNA molecules but requires to determine nucleotide sequence
    • C. does not allow comparison between DNA molecules
    • D. none of the above
    • Discuss


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