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Home Biochemistry Polymerase Chain Reaction Comments

  • Question
  • From a single molecule of DNA, PCR can make


  • Options
  • A. one additional copy
  • B. hundreds of copies
  • C. thousands of copies
  • D. millions of copies

  • Correct Answer
  • millions of copies 


  • Polymerase Chain Reaction problems


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    • 1. To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

    • Options
    • A. with the same restriction enzyme and mixed together
    • B. with different restriction enzyme and mixed together
    • C. with the combination of enzymes and then seperated
    • D. with the combination of enzymes and mixed together
    • Discuss
    • 2. In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

    • Options
    • A. 16 single strands of DNA
    • B. 16 double stranded DNA
    • C. 18 single strands of DNA
    • D. 18 double stranded DNA
    • Discuss
    • 3. PCR can be used to amplify a specific fragment of DNA from which of the following?

    • Options
    • A. A drop of blood
    • B. A hair follicle
    • C. A fragment of skin
    • D. All of these
    • Discuss
    • 4. What technique can be used to determine the murderer who left blood with the victim?

    • Options
    • A. DNA sequencing
    • B. PCR amplification
    • C. Western blot
    • D. RFLP mapping
    • Discuss
    • 5. In the presence of tryptophan in the cell, the repressor is __________

    • Options
    • A. bound to tryptophan
    • B. bound to DNA
    • C. bound to both DNA and tryptophan
    • D. bound to neither tryptophan nor DNA
    • Discuss
    • 6. PCR (Polymerase Chain Reaction) can make multiple copies of

    • Options
    • A. DNA
    • B. RNA
    • C. protein
    • D. carbohydrate
    • Discuss
    • 7. Bactriophages absorb to a bacterial surface and inject the phage DNA through the

    • Options
    • A. cell wall into cytosol
    • B. cell wall into plasmamembrane
    • C. both (a) and (b)
    • D. cell wall into endoplasmic reticulum
    • Discuss
    • 8. Additional mono saccharides are added in Golgi to produce

    • Options
    • A. either high mannose type oligosaccharide or a complex type
    • B. either fructose type monosaccharide or a complex type
    • C. either high sucrose type polysaccharide or a complex type
    • D. none of the above
    • Discuss
    • 9. Southern blotting involves

    • Options
    • A. electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
    • B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
    • C. electrophoresis of DNA molecules followed by incubation
    • D. electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
    • Discuss
    • 10. PCR is used

    • Options
    • A. to diagnose genetic diseases
    • B. to solve crimes
    • C. to study gene function
    • D. all of these
    • Discuss


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