HPLC problems
- 1. Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?
Options- A. Greater sensitivity and reusable columns
- B. Ideal for ionic species and large molecules
- C. Sample recovery
- D. All of the above Discuss
Correct Answer: All of the above
- 2. Column efficiency is measured in terms of number of plates which is
Options- A. inversely related to the square of the peak width
- B. directly related to the square of the peak width
- C. inversely related to the cube root of the peak width
- D. directly related to the square of the peak width Discuss
Correct Answer: inversely related to the square of the peak width
- 3. An isocratic elution in HPLC is one in which the composition of the solvent
Options- A. remains constant
- B. changes continuously
- C. changes in a series of steps
- D. none of these Discuss
Correct Answer: remains constant
- 4. In normal phase HPLC, there is a
Options- A. non polar solvent/polar column
- B. polar solvent/non-polar column
- C. non polar solvent/non-polar column
- D. any of the above Discuss
Correct Answer: non polar solvent/polar column
- 5. For a typical adsorbent such as silica gel, the most popular pore diameters are
Options- A. 10 and 50 A°
- B. 60 and 100 A°
- C. 100 and 150 A°
- D. 150 and 200 A° Discuss
Correct Answer: 60 and 100 A°
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