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Home Biochemistry HPLC Comments

  • Question
  • An isocratic elution in HPLC is one in which the composition of the solvent


  • Options
  • A. remains constant
  • B. changes continuously
  • C. changes in a series of steps
  • D. none of these

  • Correct Answer
  • remains constant 


  • HPLC problems


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    • 1. Column efficiency is measured in terms of number of plates which is

    • Options
    • A. inversely related to the square of the peak width
    • B. directly related to the square of the peak width
    • C. inversely related to the cube root of the peak width
    • D. directly related to the square of the peak width
    • Discuss
    • 2. Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?

    • Options
    • A. Greater sensitivity and reusable columns
    • B. Ideal for ionic species and large molecules
    • C. Sample recovery
    • D. All of the above
    • Discuss
    • 3. Dwell volume is defined as

    • Options
    • A. the volume of solvent contained in a liquid chromatographic column
    • B. the time required for the gradient to reach the column
    • C. the volume of the column between the point at which solvents are mixed and the beginning of the column
    • D. none of these
    • Discuss
    • 4. The eluent strength is a measure of

    • Options
    • A. solvent adsorption energy
    • B. solvent absorption energy
    • C. solvent diffusivity
    • D. solvent mixing index
    • Discuss
    • 5. A gradient elution in HPLC is one in which the composition of the solvent

    • Options
    • A. remains constant
    • B. is changed continuously or in a series of steps
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 6. In normal phase HPLC, there is a

    • Options
    • A. non polar solvent/polar column
    • B. polar solvent/non-polar column
    • C. non polar solvent/non-polar column
    • D. any of the above
    • Discuss
    • 7. For a typical adsorbent such as silica gel, the most popular pore diameters are

    • Options
    • A. 10 and 50 A°
    • B. 60 and 100 A°
    • C. 100 and 150 A°
    • D. 150 and 200 A°
    • Discuss
    • 8. The highly folded membranes found in such eukaryotic organelles as mitochondria and chloroplasts

    • Options
    • A. increase the surface area where key chemical processes can occur
    • B. help the cell against physical damage
    • C. make it possible to package large amounts of DNA within the cell
    • D. assist with cell movement
    • Discuss
    • 9. Lysosomes are specialized vesicles in __________ that contain digestive enzymes for the breakdown of food. A related organalle known as a vacuole, which is found in __________ , also contains enzymes but in addition may act as a storage organelle for nutrients or water.

    • Options
    • A. animals; plants and fungi
    • B. plants; animals and fungi
    • C. plants and fungi; animals
    • D. animals and plants; fungi
    • Discuss
    • 10. Which of the following is present in both prokaryotes and eukaryotes?

    • Options
    • A. Lysosome
    • B. Chloroplast
    • C. Plasma membrane
    • D. Vacuole
    • Discuss


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