Discussion
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- Question
Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?
Options- A. Greater sensitivity and reusable columns
- B. Ideal for ionic species and large molecules
- C. Sample recovery
- D. All of the above
- Correct Answer
- All of the above
- 1. Dwell volume is defined as
Options- A. the volume of solvent contained in a liquid chromatographic column
- B. the time required for the gradient to reach the column
- C. the volume of the column between the point at which solvents are mixed and the beginning of the column
- D. none of these Discuss
- 2. The eluent strength is a measure of
Options- A. solvent adsorption energy
- B. solvent absorption energy
- C. solvent diffusivity
- D. solvent mixing index Discuss
- 3. A gradient elution in HPLC is one in which the composition of the solvent
Options- A. remains constant
- B. is changed continuously or in a series of steps
- C. both (a) and (b)
- D. none of the above Discuss
- 4. In reversed phase HPLC, there is a
Options- A. non polar solvent/polar column
- B. polar solvent/non-polar column
- C. non polar solvent/non-polar column
- D. any of the above Discuss
- 5. HPLC methods include
Options- A. liquid/liquid (partition) chromatography
- B. liquid/solid (adsorption) chromatography
- C. ion exchange and size exclusion chromatography
- D. all of the above Discuss
- 6. Column efficiency is measured in terms of number of plates which is
Options- A. inversely related to the square of the peak width
- B. directly related to the square of the peak width
- C. inversely related to the cube root of the peak width
- D. directly related to the square of the peak width Discuss
- 7. An isocratic elution in HPLC is one in which the composition of the solvent
Options- A. remains constant
- B. changes continuously
- C. changes in a series of steps
- D. none of these Discuss
- 8. In normal phase HPLC, there is a
Options- A. non polar solvent/polar column
- B. polar solvent/non-polar column
- C. non polar solvent/non-polar column
- D. any of the above Discuss
- 9. For a typical adsorbent such as silica gel, the most popular pore diameters are
Options- A. 10 and 50 A°
- B. 60 and 100 A°
- C. 100 and 150 A°
- D. 150 and 200 A° Discuss
- 10. The highly folded membranes found in such eukaryotic organelles as mitochondria and chloroplasts
Options- A. increase the surface area where key chemical processes can occur
- B. help the cell against physical damage
- C. make it possible to package large amounts of DNA within the cell
- D. assist with cell movement Discuss
HPLC problems
Search Results
Correct Answer: the volume of the column between the point at which solvents are mixed and the beginning of the column
Correct Answer: solvent adsorption energy
Correct Answer: is changed continuously or in a series of steps
Correct Answer: polar solvent/non-polar column
Correct Answer: all of the above
Correct Answer: inversely related to the square of the peak width
Correct Answer: remains constant
Correct Answer: non polar solvent/polar column
Correct Answer: 60 and 100 A°
Correct Answer: increase the surface area where key chemical processes can occur
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