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Home Biochemistry HPLC Comments

  • Question
  • The eluent strength is a measure of


  • Options
  • A. solvent adsorption energy
  • B. solvent absorption energy
  • C. solvent diffusivity
  • D. solvent mixing index

  • Correct Answer
  • solvent adsorption energy 


  • HPLC problems


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    • 1. A gradient elution in HPLC is one in which the composition of the solvent

    • Options
    • A. remains constant
    • B. is changed continuously or in a series of steps
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 2. In reversed phase HPLC, there is a

    • Options
    • A. non polar solvent/polar column
    • B. polar solvent/non-polar column
    • C. non polar solvent/non-polar column
    • D. any of the above
    • Discuss
    • 3. HPLC methods include

    • Options
    • A. liquid/liquid (partition) chromatography
    • B. liquid/solid (adsorption) chromatography
    • C. ion exchange and size exclusion chromatography
    • D. all of the above
    • Discuss
    • 4. An eluotropic series

    • Options
    • A. ranks solvents by their relative abilities to displace solutes from a given absorbent
    • B. ranks column packing material by their relative abilities to retain solutes on the column
    • C. is a measure of the solvent adsorption energy
    • D. none of the above
    • Discuss
    • 5. HPLC stands for

    • Options
    • A. High Pressure Liquid Chromatography
    • B. High Performance Liquid Chromatography
    • C. both (a) and (b)
    • D. Highly Placed Liquid Chromatography
    • Discuss
    • 6. Dwell volume is defined as

    • Options
    • A. the volume of solvent contained in a liquid chromatographic column
    • B. the time required for the gradient to reach the column
    • C. the volume of the column between the point at which solvents are mixed and the beginning of the column
    • D. none of these
    • Discuss
    • 7. Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?

    • Options
    • A. Greater sensitivity and reusable columns
    • B. Ideal for ionic species and large molecules
    • C. Sample recovery
    • D. All of the above
    • Discuss
    • 8. Column efficiency is measured in terms of number of plates which is

    • Options
    • A. inversely related to the square of the peak width
    • B. directly related to the square of the peak width
    • C. inversely related to the cube root of the peak width
    • D. directly related to the square of the peak width
    • Discuss
    • 9. An isocratic elution in HPLC is one in which the composition of the solvent

    • Options
    • A. remains constant
    • B. changes continuously
    • C. changes in a series of steps
    • D. none of these
    • Discuss
    • 10. In normal phase HPLC, there is a

    • Options
    • A. non polar solvent/polar column
    • B. polar solvent/non-polar column
    • C. non polar solvent/non-polar column
    • D. any of the above
    • Discuss


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