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Home Biochemistry Protein Purification Comments

  • Question
  • Affinity chromatography deals with the


  • Options
  • A. specific binding of a protein constituents for another molecule
  • B. protein - protein interaction
  • C. protein - carbohydrate interaction
  • D. none of the above

  • Correct Answer
  • specific binding of a protein constituents for another molecule 


  • Protein Purification problems


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    • 1. Which of the following may be added to stabilize the protein after yeast cells disruption?

    • Options
    • A. NaCl
    • B. Protease inhibitor
    • C. AMP
    • D. All of these
    • Discuss
    • 2. In ion-exchange chromatography

    • Options
    • A. proteins are separated on the basis of their net charge
    • B. proteins are separated on the basis of their size
    • C. proteins are separated on the basis of their shape
    • D. either (b) or (c)
    • Discuss
    • 3. If the enthalpy change for a reaction is zero, ?G° is equal to

    • Options
    • A. T?S°
    • B. -T?S°
    • C. -?H°
    • D. lnKeq
    • Discuss
    • 4. During the unfolding reaction of a helix, breakage of each hydrogen bond requires about 2kJ/mol. This implies hydrogen bonds are

    • Options
    • A. much stronger in proteins than in water
    • B. not reformed with water
    • C. slightly weaker in proteins than in water
    • D. slightly stronger in proteins than in water
    • Discuss
    • 5. The Standard Gibb's free energy, ?G°, is

    • Options
    • A. the residual energy present in the reactants at equilibrium
    • B. the residual energy present in the products at equilibrium
    • C. the difference in the residual energy of reactants and products at equilibrium
    • D. the energy required to convert one mole of reactants to one mole of products
    • Discuss
    • 6. A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

    • Options
    • A. 1,000 units/mg
    • B. 10,000 units/mg.
    • C. 100,000 units/mg
    • D. 1,000,000 units/mg
    • Discuss
    • 7. During successful purification scheme, this may be expected that the

    • Options
    • A. specific activity increases
    • B. specific activity decreases
    • C. number of proteins in the sample decreases
    • D. both (a) and (c)
    • Discuss
    • 8. Proteins separation can be carried out on the basis of

    • Options
    • A. net charge
    • B. solubility in salt solutions
    • C. size or mass
    • D. all of these
    • Discuss
    • 9. Gel-filtration chromatography separates on the basis of

    • Options
    • A. size and shape using porous beads packed in a column
    • B. size using porous beads packed in a column
    • C. shape using porous beads packed in a column
    • D. none of the above
    • Discuss
    • 10. The best way to determine the location of protein in the purification scheme is to measure the

    • Options
    • A. rate of ATP synthesis
    • B. changes in the refractive index
    • C. UV absorption
    • D. mass spectroscopy of the protein
    • Discuss


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