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- Question
Which of the following may be added to stabilize the protein after yeast cells disruption?
Options- A. NaCl
- B. Protease inhibitor
- C. AMP
- D. All of these
- Correct Answer
- All of these
- 1. In ion-exchange chromatography
Options- A. proteins are separated on the basis of their net charge
- B. proteins are separated on the basis of their size
- C. proteins are separated on the basis of their shape
- D. either (b) or (c) Discuss
- 2. If the enthalpy change for a reaction is zero, ?G° is equal to
Options- A. T?S°
- B. -T?S°
- C. -?H°
- D. lnKeq Discuss
- 3. During the unfolding reaction of a helix, breakage of each hydrogen bond requires about 2kJ/mol. This implies hydrogen bonds are
Options- A. much stronger in proteins than in water
- B. not reformed with water
- C. slightly weaker in proteins than in water
- D. slightly stronger in proteins than in water Discuss
- 4. The Standard Gibb's free energy, ?G°, is
Options- A. the residual energy present in the reactants at equilibrium
- B. the residual energy present in the products at equilibrium
- C. the difference in the residual energy of reactants and products at equilibrium
- D. the energy required to convert one mole of reactants to one mole of products Discuss
- 5. The unfolding of regular secondary structure causes
Options- A. little increase in the entropy of protein
- B. large decrease in the entropy of the protein
- C. no change in the entropy of the protein
- D. large increase in the entropy of the protein Discuss
- 6. Affinity chromatography deals with the
Options- A. specific binding of a protein constituents for another molecule
- B. protein - protein interaction
- C. protein - carbohydrate interaction
- D. none of the above Discuss
- 7. A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
Options- A. 1,000 units/mg
- B. 10,000 units/mg.
- C. 100,000 units/mg
- D. 1,000,000 units/mg Discuss
- 8. During successful purification scheme, this may be expected that the
Options- A. specific activity increases
- B. specific activity decreases
- C. number of proteins in the sample decreases
- D. both (a) and (c) Discuss
- 9. Proteins separation can be carried out on the basis of
Options- A. net charge
- B. solubility in salt solutions
- C. size or mass
- D. all of these Discuss
- 10. Gel-filtration chromatography separates on the basis of
Options- A. size and shape using porous beads packed in a column
- B. size using porous beads packed in a column
- C. shape using porous beads packed in a column
- D. none of the above Discuss
Protein Purification problems
Search Results
Correct Answer: proteins are separated on the basis of their net charge
Correct Answer: -T?S°
Correct Answer: slightly stronger in proteins than in water
Correct Answer: the energy required to convert one mole of reactants to one mole of products
Correct Answer: large increase in the entropy of the protein
Correct Answer: specific binding of a protein constituents for another molecule
Correct Answer: 100,000 units/mg
Correct Answer: both (a) and (c)
Correct Answer: all of these
Correct Answer: size and shape using porous beads packed in a column
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