Discussion
Home ‣ Biochemistry ‣ Protein Purification Comments
- Question
A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
Options- A. 1,000 units/mg
- B. 10,000 units/mg.
- C. 100,000 units/mg
- D. 1,000,000 units/mg
- Correct Answer
- 100,000 units/mg
- 1. Affinity chromatography deals with the
Options- A. specific binding of a protein constituents for another molecule
- B. protein - protein interaction
- C. protein - carbohydrate interaction
- D. none of the above Discuss
- 2. Which of the following may be added to stabilize the protein after yeast cells disruption?
Options- A. NaCl
- B. Protease inhibitor
- C. AMP
- D. All of these Discuss
- 3. In ion-exchange chromatography
Options- A. proteins are separated on the basis of their net charge
- B. proteins are separated on the basis of their size
- C. proteins are separated on the basis of their shape
- D. either (b) or (c) Discuss
- 4. If the enthalpy change for a reaction is zero, ?G° is equal to
Options- A. T?S°
- B. -T?S°
- C. -?H°
- D. lnKeq Discuss
- 5. During the unfolding reaction of a helix, breakage of each hydrogen bond requires about 2kJ/mol. This implies hydrogen bonds are
Options- A. much stronger in proteins than in water
- B. not reformed with water
- C. slightly weaker in proteins than in water
- D. slightly stronger in proteins than in water Discuss
- 6. During successful purification scheme, this may be expected that the
Options- A. specific activity increases
- B. specific activity decreases
- C. number of proteins in the sample decreases
- D. both (a) and (c) Discuss
- 7. Proteins separation can be carried out on the basis of
Options- A. net charge
- B. solubility in salt solutions
- C. size or mass
- D. all of these Discuss
- 8. Gel-filtration chromatography separates on the basis of
Options- A. size and shape using porous beads packed in a column
- B. size using porous beads packed in a column
- C. shape using porous beads packed in a column
- D. none of the above Discuss
- 9. The best way to determine the location of protein in the purification scheme is to measure the
Options- A. rate of ATP synthesis
- B. changes in the refractive index
- C. UV absorption
- D. mass spectroscopy of the protein Discuss
- 10. The Hill coefficient (nH) for myoglobin and hemoglobin are respectively
Options- A. 2.8 and 1.0
- B. 1.0 and 2.8
- C. 1.2 and 4.5
- D. 4.5 and 1.2 Discuss
Protein Purification problems
Search Results
Correct Answer: specific binding of a protein constituents for another molecule
Correct Answer: All of these
Correct Answer: proteins are separated on the basis of their net charge
Correct Answer: -T?S°
Correct Answer: slightly stronger in proteins than in water
Correct Answer: both (a) and (c)
Correct Answer: all of these
Correct Answer: size and shape using porous beads packed in a column
Correct Answer: rate of ATP synthesis
Correct Answer: 1.0 and 2.8
Comments
There are no comments.More in Biochemistry:
Programming
Copyright ©CuriousTab. All rights reserved.