Recombinant DNA Technology problems
- 1. A plasmid
Options- A. is a circular DNA molecule
- B. always contains an origin of replication
- C. usually contains one or more restriction sites
- D. all of the above Discuss
Correct Answer: all of the above
- 2. Knockout mice are created by
Options- A. mutagenizing a mouse and selecting for mutant offspring
- B. creating a chimera by fusing cells from two different cell lines
- C. infecting the mouse with a retrovirus
- D. transfecting embryonic stem cells with an altered gene sequence Discuss
Correct Answer: transfecting embryonic stem cells with an altered gene sequence
- 3. Which of these restriction enzymes produce blunt ends?
Options- A. SaII
- B. EcoRV
- C. XhoI
- D. HindIII Discuss
Correct Answer: EcoRV
- 4. A human cDNA library
Options- A. contains DNA for specific human proteins
- B. contains DNA for virtually all of the human proteins in vectors
- C. cannot be used to obtain human genes because it would be radioactive
- D. none of the above Discuss
Correct Answer: contains DNA for virtually all of the human proteins in vectors
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