Recombinant DNA Technology problems
- 1. The unpaired nucleotides produced by the action of restriction enzymes are referred to have
Options- A. sticky ends
- B. single strands
- C. restriction fragments
- D. ligases Discuss
Correct Answer: sticky ends
- 2. The RP13 gene of chromosome 17 codes for a protein __________ .
Options- A. involved in glucose transport
- B. that is a component of hair and nails
- C. involved in eye development
- D. involved in the determination of personality Discuss
Correct Answer: involved in eye development
- 3. The order for the construction of a cDNA fragment from mRNA is to
Options- A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
- B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
- C. digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
- D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase Discuss
Correct Answer: bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
- 4. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
Options- A. in both cases molecules migrate to the anode
- B. both techniques rely on a constant charge to mass ratio
- C. both techniques utilize the sieving properties of gels
- D. all of the above Discuss
Correct Answer: all of the above
- 5. X-rays cause
Options- A. the formation of thymine dimers
- B. ionization of water in the cell
- C. heat
- D. none of the above Discuss
Correct Answer: ionization of water in the cell
- 6. Under which of the following conditions would population gene frequencies remain the same?
Options- A. Selection for homozygotes
- B. Small population size
- C. Active migration between groups
- D. Random mating Discuss
Correct Answer: Random mating
- 7. When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .
Options- A. assortative mating
- B. inbreeding
- C. heterosis
- D. genetic drift Discuss
Correct Answer: genetic drift
- 8. What enzyme forms covalent bonds between restriction fragments?
Options- A. DNA primase
- B. DNA helicase
- C. DNA polymerase
- D. DNA ligase Discuss
Correct Answer: DNA ligase
- 9. Restriction enzymes
Options- A. protect bacteria from viral infection
- B. cut DNA in a staggered fashion
- C. cut DNAs producing a blunt end
- D. all of the above Discuss
Correct Answer: all of the above
- 10. Which of the following is not true of small inbreeding populations?
Options- A. They tend to loose genetic diversity
- B. There is an increased incidence of recessive diseases
- C. Alleles may become fixed
- D. Mutation is increased Discuss
Correct Answer: Mutation is increased
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