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Home Biochemistry DNA Structure and Replication Comments

  • Question
  • In a typical electrophoresis experiment, larger fragments of DNA move more


  • Options
  • A. slowly, so they appear nearer the top of a gel photograph
  • B. quickly, so they appear nearer the top of a gel photograph
  • C. slowly, so they appear nearer the bottom of a gel photograph
  • D. quickly, so they appear nearer the bottom of a gel photograph

  • Correct Answer
  • slowly, so they appear nearer the top of a gel photograph 


  • DNA Structure and Replication problems


    Search Results


    • 1. Replication in E. coli is initiated by the generation of short RNA primers using

    • Options
    • A. RNA polymerase
    • B. primase
    • C. reverse transcriptase
    • D. both (a) and (b)
    • Discuss
    • 2. How does ethidium bromide interact with DNA?

    • Options
    • A. It binds to adenine
    • B. It binds to phosphate
    • C. It intercalates between bases
    • D. It does not interact
    • Discuss
    • 3. Which of the following is correct?

    • Options
    • A. Replication starts at a single origin
    • B. Replication is bidirectional
    • C. Replication is semi conservative
    • D. All of the above
    • Discuss
    • 4. After one replication in 15N, how many bands should be observed in a cesium chloride gradient?

    • Options
    • A. One
    • B. Two
    • C. Three
    • D. A smear
    • Discuss
    • 5. DNA polymerases I also has

    • Options
    • A. 5'-3' exonuclease activity
    • B. 3'-5'exonuclease activity
    • C. both (a) and (b)
    • D. 5'-3' endonuclease activity
    • Discuss
    • 6. One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

    • Options
    • A. Conservative theory
    • B. Dispersive theory
    • C. Semi-conservative theory
    • D. Evolutionary theory
    • Discuss
    • 7. The melting of 40-45 bp at the oriC site of E. coli requires

    • Options
    • A. DNA + ATP + HU
    • B. DNA + ATP + HU + DNAb
    • C. DNA+ ATP + HU + DNAb + DNAa
    • D. DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
    • Discuss
    • 8. In DNA replication, one new strand is synthesized complementary to an old (parent) strand and is referred as

    • Options
    • A. mutational
    • B. semiconservative
    • C. discontinuous
    • D. antiparallel
    • Discuss
    • 9. The role of primase is to

    • Options
    • A. dismantle RNA primer
    • B. cleave and unwinds short sections of DNA ahead of the replication fork
    • C. proofread base pairing
    • D. synthesize an RNA primer to begin the elongation process
    • Discuss
    • 10. The replication of a linear DNA molecule in a eukaryotic chromosome creates a problem which is reported to be sorted out by

    • Options
    • A. using enzyme called telomerase
    • B. shortening of the double stranded replicated portion
    • C. using RNA as a template
    • D. none of the above
    • Discuss


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