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- Question
Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
Options- A. positive direction
- B. 3' to 5' direction
- C. 5' to 3' direction
- D. negative direction
- Correct Answer
- 5' to 3' direction
- 1. The enzyme that joins DNA cuts is called
Options- A. joinase
- B. ligase
- C. DNA phosphorylase
- D. reverse transcriptase Discuss
- 2. The type II restriction endonucleases, bind to DNA sites
Options- A. using H-bonds for specificity
- B. that are usually palindromic
- C. using non-sequence-dependent backbone interactions
- D. all of the above Discuss
- 3. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
Options- A. Ile and Val
- B. Lys and Arg
- C. Leu and Ala
- D. Cys and Met Discuss
- 4. Cleavage of DNA by EcoRI endonuclease results in the formation of
Options- A. two 3'-OH ends and two 5'-phosphate ends
- B. one 3'-OH end and one 5'-phosphate end
- C. two 3'-phosphate ends and two 3'-OH ends
- D. one 3'-OH end and two 5'-phosphate end Discuss
- 5. Reverse transcripase from HIV does not
Options- A. uses RNA as a template
- B. has a 5' to 3' polymerase activity
- C. has a 3'- 5' exonuclease activity
- D. requires a primer Discuss
- 6. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
Options- A. Asn and Gln
- B. Cys and Met
- C. Lys and Leu
- D. Ile and Val Discuss
- 7. Which of these genes codes for a protein that plays a role in white blood cell function?
Options- A. DCP1
- B. MPO
- C. GLUT4
- D. RP13 Discuss
- 8. The TP53 gene of chromosome 17 codes for a protein __________ .
Options- A. that plays a role in the digestive process
- B. involved in glucose transport
- C. involved in the regulation of the cell cycle
- D. that is like a white blood cell protein Discuss
- 9. An example of a restriction fragment length polymorphism is
Options- A. an Eco RI cuts DNA at a different sequence than Hind III
- B. different length fragments of DNA resulting from loss or gain of a restriction site
- C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
- D. all of the above Discuss
- 10. An expression vector
Options- A. always contains an origin of replication
- B. usually contains a gene that confers antibiotic resistance to the bacterial host
- C. always contains DNA segments for the regulation of mRNA production
- D. all of the above Discuss
Protein and Nucleic Acid Interactions problems
Search Results
Correct Answer: ligase
Correct Answer: all of the above
Correct Answer: Lys and Arg
Correct Answer: two 3'-OH ends and two 5'-phosphate ends
Correct Answer: has a 3'- 5' exonuclease activity
Correct Answer: Asn and Gln
Correct Answer: MPO
Correct Answer: involved in the regulation of the cell cycle
Correct Answer: different length fragments of DNA resulting from loss or gain of a restriction site
Correct Answer: all of the above
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