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Home Biochemistry Protein and Nucleic Acid Interactions Comments

  • Question
  • The enzyme that joins DNA cuts is called


  • Options
  • A. joinase
  • B. ligase
  • C. DNA phosphorylase
  • D. reverse transcriptase

  • Correct Answer
  • ligase 


  • Protein and Nucleic Acid Interactions problems


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    • 1. The type II restriction endonucleases, bind to DNA sites

    • Options
    • A. using H-bonds for specificity
    • B. that are usually palindromic
    • C. using non-sequence-dependent backbone interactions
    • D. all of the above
    • Discuss
    • 2. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

    • Options
    • A. Ile and Val
    • B. Lys and Arg
    • C. Leu and Ala
    • D. Cys and Met
    • Discuss
    • 3. Cleavage of DNA by EcoRI endonuclease results in the formation of

    • Options
    • A. two 3'-OH ends and two 5'-phosphate ends
    • B. one 3'-OH end and one 5'-phosphate end
    • C. two 3'-phosphate ends and two 3'-OH ends
    • D. one 3'-OH end and two 5'-phosphate end
    • Discuss
    • 4. Reverse transcripase from HIV does not

    • Options
    • A. uses RNA as a template
    • B. has a 5' to 3' polymerase activity
    • C. has a 3'- 5' exonuclease activity
    • D. requires a primer
    • Discuss
    • 5. Eukaryotic mRNA binding to the ribosomes is facilitated by __________ .

    • Options
    • A. the Shine Dalgarno sequence
    • B. the 7-methyl guanosine cap
    • C. tRNA
    • D. poly A tail
    • Discuss
    • 6. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

    • Options
    • A. positive direction
    • B. 3' to 5' direction
    • C. 5' to 3' direction
    • D. negative direction
    • Discuss
    • 7. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

    • Options
    • A. Asn and Gln
    • B. Cys and Met
    • C. Lys and Leu
    • D. Ile and Val
    • Discuss
    • 8. Which of these genes codes for a protein that plays a role in white blood cell function?

    • Options
    • A. DCP1
    • B. MPO
    • C. GLUT4
    • D. RP13
    • Discuss
    • 9. The TP53 gene of chromosome 17 codes for a protein __________ .

    • Options
    • A. that plays a role in the digestive process
    • B. involved in glucose transport
    • C. involved in the regulation of the cell cycle
    • D. that is like a white blood cell protein
    • Discuss
    • 10. An example of a restriction fragment length polymorphism is

    • Options
    • A. an Eco RI cuts DNA at a different sequence than Hind III
    • B. different length fragments of DNA resulting from loss or gain of a restriction site
    • C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
    • D. all of the above
    • Discuss


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