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Home Biochemistry Protein and Nucleic Acid Interactions Comments

  • Question
  • Cleavage of DNA by EcoRI endonuclease results in the formation of


  • Options
  • A. two 3'-OH ends and two 5'-phosphate ends
  • B. one 3'-OH end and one 5'-phosphate end
  • C. two 3'-phosphate ends and two 3'-OH ends
  • D. one 3'-OH end and two 5'-phosphate end

  • Correct Answer
  • two 3'-OH ends and two 5'-phosphate ends 


  • Protein and Nucleic Acid Interactions problems


    Search Results


    • 1. Reverse transcripase from HIV does not

    • Options
    • A. uses RNA as a template
    • B. has a 5' to 3' polymerase activity
    • C. has a 3'- 5' exonuclease activity
    • D. requires a primer
    • Discuss
    • 2. Eukaryotic mRNA binding to the ribosomes is facilitated by __________ .

    • Options
    • A. the Shine Dalgarno sequence
    • B. the 7-methyl guanosine cap
    • C. tRNA
    • D. poly A tail
    • Discuss
    • 3. Protein synthesis rates in prokaryotes are limited by the rate of mRNA synthesis. If RNA synthesis occurs at the rate of 50 nucleotides/sec, then rate of protein synthesis occurs at

    • Options
    • A. 10 amino acids/sec
    • B. 17 amino acids/sec
    • C. 25 amino acids/sec
    • D. 50 amino acids/sec
    • Discuss
    • 4. Which is required for protein synthesis?

    • Options
    • A. tRNA
    • B. mRNA
    • C. rRNA
    • D. All of these
    • Discuss
    • 5. Amino acids are joined together into a protein chain by which of the following?

    • Options
    • A. Transfer RNA
    • B. DNA polymerase
    • C. Hydrogen bonds
    • D. Messenger RNA
    • Discuss
    • 6. Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

    • Options
    • A. Ile and Val
    • B. Lys and Arg
    • C. Leu and Ala
    • D. Cys and Met
    • Discuss
    • 7. The type II restriction endonucleases, bind to DNA sites

    • Options
    • A. using H-bonds for specificity
    • B. that are usually palindromic
    • C. using non-sequence-dependent backbone interactions
    • D. all of the above
    • Discuss
    • 8. The enzyme that joins DNA cuts is called

    • Options
    • A. joinase
    • B. ligase
    • C. DNA phosphorylase
    • D. reverse transcriptase
    • Discuss
    • 9. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

    • Options
    • A. positive direction
    • B. 3' to 5' direction
    • C. 5' to 3' direction
    • D. negative direction
    • Discuss
    • 10. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

    • Options
    • A. Asn and Gln
    • B. Cys and Met
    • C. Lys and Leu
    • D. Ile and Val
    • Discuss


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