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Home Biochemistry Transcription and Regulation Comments

  • Question
  • RNA polymerase in prokaryotes has a removable


  • Options
  • A. alpha subunit
  • B. beta subunit
  • C. both (a) and (b)
  • D. sigma subunit

  • Correct Answer
  • sigma subunit 


  • Transcription and Regulation problems


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    • 1. Immediately after transcription

    • Options
    • A. a methylated guanine cap is added to the 51 end of the transcript
    • B. a methylated guanine cap is added to the 31 end of the transcript
    • C. both (a) and (b)
    • D. a poly adenylation signal is added
    • Discuss
    • 2. Upstream regions which are essential for correct transcription are called

    • Options
    • A. enhancers
    • B. start codons
    • C. promoters
    • D. transcription factors
    • Discuss
    • 3. The genetic code is

    • Options
    • A. universal
    • B. universal except for rare exceptions in mitochondria and some protozoa
    • C. species-specific
    • D. kingdom-specific
    • Discuss
    • 4. Crick demonstrated that the genetic code involved three bases and suggested that the code was degenerated. What experimental technique Crick conducted to suggest genetic code degeneration?

    • Options
    • A. Gel electrophoresis
    • B. Density gradient centrifugation
    • C. Frameshift mutagenesis
    • D. Restriction digests of the rII gene
    • Discuss
    • 5. Codon that specify the amino acids often differs in the

    • Options
    • A. first base
    • B. second base
    • C. third base
    • D. none of these
    • Discuss
    • 6. Transcription factors are

    • Options
    • A. promoters
    • B. proteins which bind to DNA and regulate transcription
    • C. TATA boxes
    • D. CATT boxes
    • Discuss
    • 7. Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?

    • Options
    • A. Presence of glucose in the growth media
    • B. Presence of lactose in the growth media
    • C. Presence of IPTG (isopropyl thiogalactoside) in the growth media
    • D. Both (b) and (c)
    • Discuss
    • 8. Rho-dependent termination of transcription in E. coli

    • Options
    • A. requires ATP
    • B. requires about 50 nucleotides of uncomplexed mRNA
    • C. both (a) and (b)
    • D. removes mRNA and holoenzyme from the DNA
    • Discuss
    • 9. The transcriptional regulatory proteins in eukaryotes which induce transcription usually have

    • Options
    • A. at least two distinct domains of protein structure, a DNA binding domain and an activation domain
    • B. at least two distinct domains of protein structure and a DNA binding domain
    • C. at least one distinct domains of protein structure, a DNA binding domain and an activation domain
    • D. none of these
    • Discuss
    • 10. The closed complex at bacterial promoters is

    • Options
    • A. in equilibrium with free RNA polymerase and the promoter
    • B. not affected by promoter mutations
    • C. bound more tightly when repressors are present
    • D. not affected by activator proteins
    • Discuss


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