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Home Biochemistry Transcription and Regulation Comments

  • Question
  • The transcriptional regulatory proteins in eukaryotes which induce transcription usually have


  • Options
  • A. at least two distinct domains of protein structure, a DNA binding domain and an activation domain
  • B. at least two distinct domains of protein structure and a DNA binding domain
  • C. at least one distinct domains of protein structure, a DNA binding domain and an activation domain
  • D. none of these

  • Correct Answer
  • at least two distinct domains of protein structure, a DNA binding domain and an activation domain 


  • Transcription and Regulation problems


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    • 1. Rho-dependent termination of transcription in E. coli

    • Options
    • A. requires ATP
    • B. requires about 50 nucleotides of uncomplexed mRNA
    • C. both (a) and (b)
    • D. removes mRNA and holoenzyme from the DNA
    • Discuss
    • 2. Which of the following conditions would cause the release of the lac repressor protein from the lac operator site on DNA?

    • Options
    • A. Presence of glucose in the growth media
    • B. Presence of lactose in the growth media
    • C. Presence of IPTG (isopropyl thiogalactoside) in the growth media
    • D. Both (b) and (c)
    • Discuss
    • 3. Transcription factors are

    • Options
    • A. promoters
    • B. proteins which bind to DNA and regulate transcription
    • C. TATA boxes
    • D. CATT boxes
    • Discuss
    • 4. RNA polymerase in prokaryotes has a removable

    • Options
    • A. alpha subunit
    • B. beta subunit
    • C. both (a) and (b)
    • D. sigma subunit
    • Discuss
    • 5. Immediately after transcription

    • Options
    • A. a methylated guanine cap is added to the 51 end of the transcript
    • B. a methylated guanine cap is added to the 31 end of the transcript
    • C. both (a) and (b)
    • D. a poly adenylation signal is added
    • Discuss
    • 6. The closed complex at bacterial promoters is

    • Options
    • A. in equilibrium with free RNA polymerase and the promoter
    • B. not affected by promoter mutations
    • C. bound more tightly when repressors are present
    • D. not affected by activator proteins
    • Discuss
    • 7. The complex of RNA polymerase, DNA template and new RNA transcript is called

    • Options
    • A. transcription bubble
    • B. replication bubble
    • C. a translation bubble
    • D. none of these
    • Discuss
    • 8. The binding of lac repressor to DNA could be considered to be analogous to

    • Options
    • A. competitive inhibition of an enzyme
    • B. mixed-type inhibition of an enzyme
    • C. uncompetitive inhibition of an enzyme
    • D. allosteric effects in enzyme regulation
    • Discuss
    • 9. Enhancers are regions that

    • Options
    • A. bind RNA polymerase
    • B. are adjacent to the TATA box
    • C. are CAT box binding proteins
    • D. modulate transcription
    • Discuss
    • 10. Transcription results in

    • Options
    • A. an amino acid chain
    • B. messenger RNA
    • C. complementary DNA
    • D. Okazaki fragments
    • Discuss


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