Immunological Techniques problems
- 1. Which of the following radioactive element is generally involved in RIA?
Options- A. Tritium
- B. Carbon-14
- C. Iodine-125
- D. All of these Discuss
Correct Answer: All of these
- 2. Radial Immunodiffusion is similar to
Options- A. double-diffusion
- B. gel diffusion
- C. Ouchterloney technique
- D. all of these Discuss
Correct Answer: double-diffusion
- 3. The IPTG is a gratuitous inducers of (?-galactosidase, and x-gal is a chromogenic substrate. When ?-complementation occurs which colour is produced?
Options- A. Blue
- B. White
- C. Brown
- D. No colour is produced Discuss
Correct Answer: Blue
- 4. Which of the following is an immunodiffusion test?
Options- A. Double-diffusion
- B. Gel diffusion
- C. Ouchterloney technique
- D. All of these Discuss
Correct Answer: All of these
- 5. An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
Options- A. there was more than one antigen
- B. the antibody was impure
- C. the temperature was too high
- D. there was one antibody and one antigen Discuss
Correct Answer: there was more than one antigen
- 6. Which of the following is an immunoelectrophoresis technique?
Options- A. Two-dimensional immunoelectrophoresis
- B. Counterimmunoelectrophoresis
- C. Coimmunoelectrophoresis
- D. Both (a) and (b) Discuss
Correct Answer: Both (a) and (b)
- 7. Immunoaffinity chromatography can be used in biochemical applications to
Options- A. break down antibody structure
- B. purify protein antigen
- C. break down antigen and analyze quantitatively
- D. none of the above Discuss
Correct Answer: purify protein antigen
- 8. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
Options- A. The ELISA would not develop when the substrate was added
- B. The ELISA would develop normally
- C. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
- D. None of the above Discuss
Correct Answer: All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
- 9. In Rocket Immunodiffusion the length of the rocket is
Options- A. proportional to the amount of antibody placed in each well
- B. inversely proportional to the amount of antibody placed in each well
- C. inversely proportional to the amount of antigen placed in each well
- D. proportional to the amount of antigen placed in each well Discuss
Correct Answer: proportional to the amount of antigen placed in each well
- 10. Agarose a porous matrix used in various molecular biology technique is made by repetitive units of
Options- A. maltose
- B. agarobiose
- C. agar
- D. none of these Discuss
Correct Answer: maltose
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