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Biochemistry
‣
Gas Chromatography
Comments
Question
Column bleeding occurs when
Options
A. elution of the analyte is extended over time
B. the column is cracked and stationary phase leaks out
C. traces of the stationary phase are eluted
D. the column breaks during installation and causes personal injury
Correct Answer
traces of the stationary phase are eluted
Gas Chromatography problems
Search Results
1. Which of these effects result from slow injection of a large sample volume?
Options
A. Increased resolution
B. Decreased resolution
C. Non-linear detector response
D. Constant resolution
Show Answer
Scratch Pad
Discuss
Correct Answer: Decreased resolution
2. Which of the following is not used for detection in GC?
Options
A. Infrared spectroscopy
B. NMR
C. Flame ionisation
D. Electrical conductivity
Show Answer
Scratch Pad
Discuss
Correct Answer: NMR
3. Which of the following detectors give mass flow-dependent signals?
Options
A. Electron capture detector
B. Field ionisation detector
C. Thermal conductivity detector
D. All of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: Field ionisation detector
4. The column is heated to
Options
A. prevent analyte condensation within the column
B. control elution of the different analytes
C. reduce band broadening to get sharper peaks
D. all of these
Show Answer
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Discuss
Correct Answer: all of these
5. Resolution is proportional to the
Options
A. number of theoretical plates in a column
B. square root of the number of theoretical plates in a column
C. square of the number of theoretical plates in a column
D. cube root of the number of theoretical plates in a column
Show Answer
Scratch Pad
Discuss
Correct Answer: square root of the number of theoretical plates in a column
6. What does the selectivity factor describe?
Options
A. The proportional difference in widths of two chromatographic peaks
B. The maximum number of different species which a column can separate simultaneously
C. The relative separation achieved between two species
D. None of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: The relative separation achieved between two species
7. Helium is generally preferred as carrier gas over nitrogen and hydrogen because
Options
A. it is inert
B. it has a lower viscosity
C. it doubles up as a party gas for balloons and funny voices
D. all of above
Show Answer
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Discuss
Correct Answer: all of above
8. Doubling the column's length increases resolution by a factor of
Options
A. (2)
0.5
B. 2
C. 3
D. 4
Show Answer
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Discuss
Correct Answer: (2)
0.5
9. The DNA polymerase in bacteria, responsible for DNA synthesis is
Options
A. Polymerase I
B. Polymerase II
C. Polymerase III
D. all of these
Show Answer
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Discuss
Correct Answer: Polymerase III
10. Which of the following are short nucleotides strands made by DNA polymerase working in the opposite direction of the replication fork?
Options
A. Leading strands
B. Okazaki fragments
C. Primers
D. Lagging strand
Show Answer
Scratch Pad
Discuss
Correct Answer: Okazaki fragments
Comments
ppu-prof_mr
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