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Biochemistry
‣
Gas Chromatography
Comments
Question
The GC trace obtained after an experiment is called a
Options
A. chromatograph
B. chromatogram
C. chromatophore
D. graph
Correct Answer
chromatogram
Gas Chromatography problems
Search Results
1. What useful information can be found from a Van Deemter plot?
Options
A. The selectivity factor
B. Optimum mobile phase flow rate
C. Optimum column temperature
D. Optimum column length
Show Answer
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Discuss
Correct Answer: Optimum mobile phase flow rate
2. Split injection is carried out by
Options
A. splitting the sample into smaller portions to inject sequentially
B. splitting the sample into smaller portions to inject at the same time through parallel ports
C. splitting off some of the sample so that it does not enter the column
D. none of the above
Show Answer
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Correct Answer: splitting off some of the sample so that it does not enter the column
3. A retention gap is placed between the injector and the front of the column to
Options
A. retain contaminants and prevent them from reaching the column
B. retain the sample and release it gradually to the column
C. prevent backflush of the injected solution
D. all of the above
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Correct Answer: retain contaminants and prevent them from reaching the column
4. What is the typical internal diameter of fused silica capillary columns?
Options
A. 0.2-0.3 mm
B. 0.3-0.5mm
C. 0.5-1.0 mm
D. 1.0-2.0 mm
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Correct Answer: 0.2-0.3 mm
5. Theoretical plates are used to
Options
A. estimate the efficiency of a column
B. determine the thickness of the stationary phase
C. measure the distribution of the analyte between mobile and stationary phases
D. None of the above
Show Answer
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Correct Answer: estimate the efficiency of a column
6. Which of the statements is correct?
Options
A. Gas chromatography is used to analyse gases
B. Gas chromatography is used to analyse solids
C. Gas chromatography is used to analyse gases, solutions and solids
D. All of the above
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Correct Answer: All of the above
7. What does the retention factor,
k'
, describe?
Options
A. The distribution of an analyte between the stationary and the mobile phase
B. The migration rate of an analyte through a column
C. The velocity of the mobile phase
D. All of these
Show Answer
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Correct Answer: The migration rate of an analyte through a column
8. Sample retention in the column is measured by
Options
A. retention time
B. retention factor
C. retention index
D. all of these
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Correct Answer: all of these
9. Sample injection is considered successful if
Options
A. all of the sample in the injector has been added to the column
B. the sample is concentrated at the start of the column
C. the sample is spread evenly along the column
D. the sample is homogenously spread along the column
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Correct Answer: the sample is concentrated at the start of the column
10. What are the benefits of decreasing the column internal diameter?
Options
A. Increased sample capacity
B. Increased resolution
C. Reduced risk of column overloading
D. All of the above
Show Answer
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Correct Answer: Increased resolution
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