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Home Biochemistry FT IR Spectroscopy Comments

  • Question
  • Cytochrome C has an isoelectric pH of


  • Options
  • A. 8.5
  • B. 10.05
  • C. 7.5
  • D. 11.05

  • Correct Answer
  • 10.05 


  • FT IR Spectroscopy problems


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    • 1. When placed in a magnetic field, all the random spins of the nuclei

    • Options
    • A. stop
    • B. reverse direction
    • C. align with the magnetic field
    • D. rotate to 90° away from the induced field
    • Discuss
    • 2. Why is it important to use a deuterated solvent?

    • Options
    • A. NMR uses least of this solvent
    • B. So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
    • C. Expensive solvents work best with NMR
    • D. They dissolve polymer the fastest
    • Discuss
    • 3. Coupling causes the peaks in 1H NMR spectra to be split into

    • Options
    • A. two peaks
    • B. multiple peaks equal to the number of hydrogens on surrounding atoms
    • C. multiple peaks equal to the number of surrounding carbon atoms
    • D. multiple peaks equal to the number of hydrogen on surrounding atoms, plus one
    • Discuss
    • 4. Better understanding of the nuclei is possible,

    • Options
    • A. with the help of wavelength spectrum
    • B. with the help of frequencies ranges
    • C. with the help of a mathematical translator called the fourier transfer algorithm
    • D. none of the above
    • Discuss
    • 5. What is used to cool the superconducting coil?

    • Options
    • A. Hydrogen
    • B. Ice
    • C. Dry ice
    • D. Liquid helium
    • Discuss
    • 6. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?

    • Options
    • A. Molecular mass
    • B. Solubility
    • C. Isoelectric point
    • D. Folding
    • Discuss
    • 7. How do you turn a signal recorded in the time domain into a frequency domain signal?

    • Options
    • A. Fourier transformation
    • B. Measurement of peak areas
    • C. By use of a Michelson interferometer
    • D. None of the above
    • Discuss
    • 8. Negative staining is a technique used in

    • Options
    • A. electron microscopy
    • B. gel electrophoresis
    • C. immunocytochemistry
    • D. light microscopy
    • Discuss
    • 9. In scanning electron microscopy

    • Options
    • A. a specimen is fixed and then coated with thin layer of a heavy metal
    • B. a specimen is fixed and then coated with transition metal
    • C. a specimen is not fixed and then coated with thin layer of a heavy metal
    • D. none of the above
    • Discuss
    • 10. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?

    • Options
    • A. 2
    • B. 4
    • C. 3
    • D. 6
    • Discuss


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