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Home Biochemistry FT IR Spectroscopy Comments

  • Question
  • Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?


  • Options
  • A. Molecular mass
  • B. Solubility
  • C. Isoelectric point
  • D. Folding

  • Correct Answer
  • Isoelectric point 


  • FT IR Spectroscopy problems


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    • 1. Cytochrome C has an isoelectric pH of

    • Options
    • A. 8.5
    • B. 10.05
    • C. 7.5
    • D. 11.05
    • Discuss
    • 2. When placed in a magnetic field, all the random spins of the nuclei

    • Options
    • A. stop
    • B. reverse direction
    • C. align with the magnetic field
    • D. rotate to 90° away from the induced field
    • Discuss
    • 3. Why is it important to use a deuterated solvent?

    • Options
    • A. NMR uses least of this solvent
    • B. So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
    • C. Expensive solvents work best with NMR
    • D. They dissolve polymer the fastest
    • Discuss
    • 4. Coupling causes the peaks in 1H NMR spectra to be split into

    • Options
    • A. two peaks
    • B. multiple peaks equal to the number of hydrogens on surrounding atoms
    • C. multiple peaks equal to the number of surrounding carbon atoms
    • D. multiple peaks equal to the number of hydrogen on surrounding atoms, plus one
    • Discuss
    • 5. Better understanding of the nuclei is possible,

    • Options
    • A. with the help of wavelength spectrum
    • B. with the help of frequencies ranges
    • C. with the help of a mathematical translator called the fourier transfer algorithm
    • D. none of the above
    • Discuss
    • 6. How do you turn a signal recorded in the time domain into a frequency domain signal?

    • Options
    • A. Fourier transformation
    • B. Measurement of peak areas
    • C. By use of a Michelson interferometer
    • D. None of the above
    • Discuss
    • 7. Negative staining is a technique used in

    • Options
    • A. electron microscopy
    • B. gel electrophoresis
    • C. immunocytochemistry
    • D. light microscopy
    • Discuss
    • 8. In scanning electron microscopy

    • Options
    • A. a specimen is fixed and then coated with thin layer of a heavy metal
    • B. a specimen is fixed and then coated with transition metal
    • C. a specimen is not fixed and then coated with thin layer of a heavy metal
    • D. none of the above
    • Discuss
    • 9. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?

    • Options
    • A. 2
    • B. 4
    • C. 3
    • D. 6
    • Discuss
    • 10. In FT-NMR, how are nuclei excited?

    • Options
    • A. By radio-frequency radiation whose frequency is swept across a predetermined range
    • B. By an intense pulse of radiation which contains a wide range of frequencies
    • C. By an intense pressure
    • D. None of the above
    • Discuss


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