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Biochemistry
‣
FT IR Spectroscopy
Comments
Question
Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
Options
A. Molecular mass
B. Solubility
C. Isoelectric point
D. Folding
Correct Answer
Isoelectric point
FT IR Spectroscopy problems
Search Results
1. Cytochrome C has an isoelectric pH of
Options
A. 8.5
B. 10.05
C. 7.5
D. 11.05
Show Answer
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Discuss
Correct Answer: 10.05
2. When placed in a magnetic field, all the random spins of the nuclei
Options
A. stop
B. reverse direction
C. align with the magnetic field
D. rotate to 90° away from the induced field
Show Answer
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Discuss
Correct Answer: align with the magnetic field
3. Why is it important to use a deuterated solvent?
Options
A. NMR uses least of this solvent
B. So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
C. Expensive solvents work best with NMR
D. They dissolve polymer the fastest
Show Answer
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Discuss
Correct Answer: So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
4. Coupling causes the peaks in
1
H NMR spectra to be split into
Options
A. two peaks
B. multiple peaks equal to the number of hydrogens on surrounding atoms
C. multiple peaks equal to the number of surrounding carbon atoms
D. multiple peaks equal to the number of hydrogen on surrounding atoms, plus one
Show Answer
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Correct Answer: multiple peaks equal to the number of hydrogen on surrounding atoms, plus one
5. Better understanding of the nuclei is possible,
Options
A. with the help of wavelength spectrum
B. with the help of frequencies ranges
C. with the help of a mathematical translator called the fourier transfer algorithm
D. none of the above
Show Answer
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Correct Answer: with the help of a mathematical translator called the fourier transfer algorithm
6. How do you turn a signal recorded in the time domain into a frequency domain signal?
Options
A. Fourier transformation
B. Measurement of peak areas
C. By use of a Michelson interferometer
D. None of the above
Show Answer
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Discuss
Correct Answer: Fourier transformation
7. Negative staining is a technique used in
Options
A. electron microscopy
B. gel electrophoresis
C. immunocytochemistry
D. light microscopy
Show Answer
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Discuss
Correct Answer: electron microscopy
8. In scanning electron microscopy
Options
A. a specimen is fixed and then coated with thin layer of a heavy metal
B. a specimen is fixed and then coated with transition metal
C. a specimen is not fixed and then coated with thin layer of a heavy metal
D. none of the above
Show Answer
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Discuss
Correct Answer: a specimen is fixed and then coated with thin layer of a heavy metal
9. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
Options
A. 2
B. 4
C. 3
D. 6
Show Answer
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Discuss
Correct Answer: 2
10. In FT-NMR, how are nuclei excited?
Options
A. By radio-frequency radiation whose frequency is swept across a predetermined range
B. By an intense pulse of radiation which contains a wide range of frequencies
C. By an intense pressure
D. None of the above
Show Answer
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Discuss
Correct Answer: By radio-frequency radiation whose frequency is swept across a predetermined range
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Recombinant DNA Technology
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