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Home Biochemistry Gel Electrophoresis See What Others Are Saying!
  • Question
  • In isoelectric focusing, proteins are separated on the basis of their


  • Options
  • A. relative content of positively charged residue only
  • B. relative content of negatively charged residue only
  • C. size
  • D. relative content of positively and negatively charged residue

  • Correct Answer
  • relative content of positively and negatively charged residue 


  • More questions

    • 1. Which of the following indicates that the pK of an acid is numerically equal to the pH of the solution when the molar concentration of the acid and its conjugate base are equal?

    • Options
    • A. Michaelis-Menten equation
    • B. Haldanes equation
    • C. Henderson-Hasselbalch equation
    • D. Hardy-Windberg law
    • Discuss
    • 2. Which of the following acid/base pairs act as natural buffers in living systems?

    • Options
    • A. H2CO3/HCO3-
    • B. H2PO4-/HPO42-
    • C. Histidine+/histidine
    • D. All of these
    • Discuss
    • 3. Which of the following statements about covalently bonded molecules is false?

    • Options
    • A. Electrons are shared between the atoms that make up the molecule
    • B. Noncovalent bonds may also be present, especially if the molecule is large
    • C. Such molecules often result from the interaction of ionized atoms
    • D. The chemical bonds that hold the molecule together are relatively strong
    • Discuss
    • 4. Secondary structure in protein refers to

    • Options
    • A. linear sequence of amino acids joined together by peptide bond
    • B. three dimensional arrangement of all amino acids in polypeptide chain
    • C. regular folding of regions of the polypeptide chain
    • D. protein made up of more than one polypeptide chain
    • Discuss
    • 5. The specificity of a ligand binding site on a protein is based on

    • Options
    • A. the absence of competing ligands
    • B. the amino acid residues lining the binding site
    • C. the presence of hydrating water molecules
    • D. the opposite chirality of the binding ligand
    • Discuss
    • 6. Hydrogen bonds and hydrophilic interactions are types of

    • Options
    • A. weak chemical bonds that hold together the atoms within a molecule
    • B. strong chemical bonds that hold together the atoms within a molecule
    • C. weak chemical bonds that link together separate molecules
    • D. strong chemical bonds that link together separate molecules
    • Discuss
    • 7. Internal conversion is where

    • Options
    • A. the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)
    • B. A molecule converts its excess energy to light, and emits a photon
    • C. A molecule converts excess electronic energy to vibrational energy
    • D. none of the above
    • Discuss
    • 8. Who discovered and described the blood groups (ABO) classification?

    • Options
    • A. Theodor Kocher
    • B. Karl Landsteiner
    • C. Otto Warburg
    • D. Karl Hooper
    • Discuss
    • 9. Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

    • Options
    • A. prior to reaction with antibody
    • B. prior to reaction with antigen
    • C. after reaction with antibody
    • D. after reaction with antigen
    • Discuss
    • 10. Processive synthesis is a characteristic feature of

    • Options
    • A. all DNA polymerases.
    • B. DNA Pol III at a replication fork.
    • C. removal of RNA primers on Okasaki fragments.
    • D. DNA mismatch repair
    • Discuss


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