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- Question
Void volume refers to the
Options- A. total volume of eluent in the column the remainder being taken up by the packing material
- B. the volume of solvent contained in a liquid chromatographic column
- C. the time required for the gradient to reach the column
- D. the volume of the column between the point at which solvents are mixed and the beginning of the column
- Correct Answer
- total volume of eluent in the column the remainder being taken up by the packing material
- 1. Which can be used as a mobile phase in HPLC applications?
Options- A. Any compound with solubility in liquid
- B. Any compound with limited solubility in liquid
- C. Any compound with non-solubility in liquid
- D. Any of the above Discuss
- 2. Which of the following statements is true for a refractive index detector in HPLC?
Options- A. It is more sensitive than a UV detector
- B. It can only be used for isocratic elutions
- C. It does not respond to many solutes
- D. none of above Discuss
- 3. A combination of paper chromatography and electrophoresis involves
Options- A. partition chromatography
- B. electrical mobility of the ionic species
- C. both (a) and (b)
- D. none of these Discuss
- 4. Thin layer chromatography is
Options- A. partition chromatography
- B. electrical mobility of ionic species
- C. adsorption chromatography
- D. none of the above Discuss
- 5. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)
Options- A. V = V0 + kDVi
- B. V = V0/Vi
- C. V = V0 - kDVi
- D. V/V0 = kDVi Discuss
- 6. HPLC stands for
Options- A. High Pressure Liquid Chromatography
- B. High Performance Liquid Chromatography
- C. both (a) and (b)
- D. Highly Placed Liquid Chromatography Discuss
- 7. An eluotropic series
Options- A. ranks solvents by their relative abilities to displace solutes from a given absorbent
- B. ranks column packing material by their relative abilities to retain solutes on the column
- C. is a measure of the solvent adsorption energy
- D. none of the above Discuss
- 8. HPLC methods include
Options- A. liquid/liquid (partition) chromatography
- B. liquid/solid (adsorption) chromatography
- C. ion exchange and size exclusion chromatography
- D. all of the above Discuss
- 9. In reversed phase HPLC, there is a
Options- A. non polar solvent/polar column
- B. polar solvent/non-polar column
- C. non polar solvent/non-polar column
- D. any of the above Discuss
- 10. A gradient elution in HPLC is one in which the composition of the solvent
Options- A. remains constant
- B. is changed continuously or in a series of steps
- C. both (a) and (b)
- D. none of the above Discuss
HPLC problems
Search Results
Correct Answer: Any compound with solubility in liquid
Correct Answer: It does not respond to many solutes
Correct Answer: both (a) and (b)
Correct Answer: adsorption chromatography
Correct Answer: V = V0 + kDVi
Correct Answer: both (a) and (b)
Correct Answer: ranks solvents by their relative abilities to displace solutes from a given absorbent
Correct Answer: all of the above
Correct Answer: polar solvent/non-polar column
Correct Answer: is changed continuously or in a series of steps
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