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Home Biochemistry Polymerase Chain Reaction Comments

  • Question
  • PCR amplifies millions of copies of


  • Options
  • A. a short fragment of DNA
  • B. the entire genome (all the DNA in a sample)
  • C. both (a) and (b)
  • D. short chain RNA

  • Correct Answer
  • a short fragment of DNA 


  • Polymerase Chain Reaction problems


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    • 1. The viruses that can achieve neoplastic transformation are called

    • Options
    • A. DNA tumor viruses
    • B. RNA tumor viruses
    • C. retroviruses HIV
    • D. none of these
    • Discuss
    • 2. A PCR cycle consists of

    • Options
    • A. three steps, denaturation, primer annealing and elongation
    • B. three steps, denaturation, initiation and elongation
    • C. three steps, primer annealing, elongation and termination
    • D. three steps, initiation, elongation and termination
    • Discuss
    • 3. Restriction maps

    • Options
    • A. allows comparison between DNA molecules without the need to determine nucleotide sequence
    • B. allows comparison between DNA molecules but requires to determine nucleotide sequence
    • C. does not allow comparison between DNA molecules
    • D. none of the above
    • Discuss
    • 4. Which of the following is correct?

    • Options
    • A. HIV is a retrovirus that kills human helper T cells
    • B. Causes acquired immune deficiency syndrome (AIDS)
    • C. Cripples the immunity systems
    • D. All of the above
    • Discuss
    • 5. Genomic libraries are made from

    • Options
    • A. genomic DNA of an organism
    • B. genomic RNA of an organism
    • C. genomic cDNA of an organism
    • D. genomic mRNA of an organism
    • Discuss
    • 6. Transformation means

    • Options
    • A. formation of a pilus
    • B. acquiring DNA from the bacterial cell environment
    • C. plasmid containing a F factor
    • D. F+ and F- strains of bacteria
    • Discuss
    • 7. PCR can be used in

    • Options
    • A. cloning
    • B. sequencing
    • C. medical diagnosis and forensic medicine
    • D. all of the above
    • Discuss
    • 8. How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?

    • Options
    • A. Two ds DNA molecule
    • B. Three ds DNA molecule
    • C. Eight ds DNA molecule
    • D. Four ds DNA molecule
    • Discuss
    • 9. PCR is an abbreviation for

    • Options
    • A. Protein Change Reaction
    • B. Polymerase Chain Reaction
    • C. Positive Charge Reaction:
    • D. Preliminary Change Rate
    • Discuss
    • 10. DNA fragments in a restriction digest can be separated by electrophoresis in

    • Options
    • A. poly acrylamide
    • B. agarose gel
    • C. both (a) and (b)
    • D. none of these
    • Discuss


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