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Home Biochemistry Polymerase Chain Reaction Comments

  • Question
  • Restriction maps


  • Options
  • A. allows comparison between DNA molecules without the need to determine nucleotide sequence
  • B. allows comparison between DNA molecules but requires to determine nucleotide sequence
  • C. does not allow comparison between DNA molecules
  • D. none of the above

  • Correct Answer
  • allows comparison between DNA molecules without the need to determine nucleotide sequence 


  • Polymerase Chain Reaction problems


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    • 1. Which of the following is correct?

    • Options
    • A. HIV is a retrovirus that kills human helper T cells
    • B. Causes acquired immune deficiency syndrome (AIDS)
    • C. Cripples the immunity systems
    • D. All of the above
    • Discuss
    • 2. Genomic libraries are made from

    • Options
    • A. genomic DNA of an organism
    • B. genomic RNA of an organism
    • C. genomic cDNA of an organism
    • D. genomic mRNA of an organism
    • Discuss
    • 3. Specialized transduction occurs when

    • Options
    • A. the bacteriophage incorporates randomly in the bacterial chromosome
    • B. the bacteriophage never incorporates into the bacterial chromosome
    • C. the bacteriophage always incorporates at the same position in the bacterial chromosome
    • D. none of the above
    • Discuss
    • 4. Double stranded DNA denaturation with specified limit of temperature is

    • Options
    • A. reversible reaction
    • B. irreversible reaction
    • C. either (a) or (b)
    • D. none of these
    • Discuss
    • 5. PCR is used

    • Options
    • A. to diagnose genetic diseases
    • B. to solve crimes
    • C. to study gene function
    • D. all of these
    • Discuss
    • 6. A PCR cycle consists of

    • Options
    • A. three steps, denaturation, primer annealing and elongation
    • B. three steps, denaturation, initiation and elongation
    • C. three steps, primer annealing, elongation and termination
    • D. three steps, initiation, elongation and termination
    • Discuss
    • 7. The viruses that can achieve neoplastic transformation are called

    • Options
    • A. DNA tumor viruses
    • B. RNA tumor viruses
    • C. retroviruses HIV
    • D. none of these
    • Discuss
    • 8. PCR amplifies millions of copies of

    • Options
    • A. a short fragment of DNA
    • B. the entire genome (all the DNA in a sample)
    • C. both (a) and (b)
    • D. short chain RNA
    • Discuss
    • 9. Transformation means

    • Options
    • A. formation of a pilus
    • B. acquiring DNA from the bacterial cell environment
    • C. plasmid containing a F factor
    • D. F+ and F- strains of bacteria
    • Discuss
    • 10. PCR can be used in

    • Options
    • A. cloning
    • B. sequencing
    • C. medical diagnosis and forensic medicine
    • D. all of the above
    • Discuss


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