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Biochemistry
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Immunological Techniques
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Question
DNA- footprinting is a suitable technique for identifying which of the following?
Options
A. Particular mRNA in mixture
B. Particular t-RNA in mixture
C. Introns within DNA
D. Protein binding site within DNA
Correct Answer
Protein binding site within DNA
Immunological Techniques problems
Search Results
1. In Counter immunoelectrophoresis
Options
A. electrophoresis will drive the antibody and antigen parallel to each other
B. electrophoresis will drive the antibody and antigen toward each other
C. the antibody will migrate towards anode
D. the antibody will migrate towards cathode
Show Answer
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Discuss
Correct Answer: electrophoresis will drive the antibody and antigen toward each other
2. Agarose a porous matrix used in various molecular biology technique is made by repetitive units of
Options
A. maltose
B. agarobiose
C. agar
D. none of these
Show Answer
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Correct Answer: maltose
3. In Rocket Immunodiffusion the length of the rocket is
Options
A. proportional to the amount of antibody placed in each well
B. inversely proportional to the amount of antibody placed in each well
C. inversely proportional to the amount of antigen placed in each well
D. proportional to the amount of antigen placed in each well
Show Answer
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Correct Answer: proportional to the amount of antigen placed in each well
4. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
Options
A. The ELISA would not develop when the substrate was added
B. The ELISA would develop normally
C. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
D. None of the above
Show Answer
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Discuss
Correct Answer: All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
5. Immunoaffinity chromatography can be used in biochemical applications to
Options
A. break down antibody structure
B. purify protein antigen
C. break down antigen and analyze quantitatively
D. none of the above
Show Answer
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Correct Answer: purify protein antigen
6. Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis
Options
A. prior to reaction with antibody
B. prior to reaction with antigen
C. after reaction with antibody
D. after reaction with antigen
Show Answer
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Correct Answer: prior to reaction with antibody
7. The movement of molecules across a membrane by passive transport does not require
Options
A. an input of metabolic energy
B. internal energy
C. vibrational energy
D. none of these
Show Answer
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Correct Answer: an input of metabolic energy
8. Which of the following is correct?
Options
A. Membranes form boundaries around the cell and distinct subcellular components
B. Membranes act as selectively permeable barrier
C. Membranes contain varying amount of lipid and protein and some contain small amount of carbohydrates
D. All of the above
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Correct Answer: All of the above
9. The mesosomes may be the sites of
Options
A. DNA replication and specialized enzymatic reactions
B. RNA replications and specialized enzymatic reactions
C. RNA replications and specialized cyclic reactions
D. None of the above
Show Answer
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Correct Answer: DNA replication and specialized enzymatic reactions
10. Which type of molecule spans the membrane, from its inner to outer surface according to the Fluid mosaic model of cell membranes?
Options
A. Cholesterol
B. Phospholipid
C. Protein
D. Carbohydrate
Show Answer
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Discuss
Correct Answer: Protein
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Allosteric Effects
Amino Acid Metabolism
Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
Gel Electrophoresis
Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Immunological Techniques
Lipid
Membrane Structure and Functions
Minerals
Nitrogen Metabolism
NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
Structure and Properties of Peptides
TCA Cycle
Thermodynamics and Free Energy
Transcription and Regulation
UV Luminance Spectroscopy
Vitamins and Coenzymes
Water, pH and Macromolecules