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Biochemistry
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Immunological Techniques
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Question
Agarose a porous matrix used in various molecular biology technique is made by repetitive units of
Options
A. maltose
B. agarobiose
C. agar
D. none of these
Correct Answer
maltose
Immunological Techniques problems
Search Results
1. In Rocket Immunodiffusion the length of the rocket is
Options
A. proportional to the amount of antibody placed in each well
B. inversely proportional to the amount of antibody placed in each well
C. inversely proportional to the amount of antigen placed in each well
D. proportional to the amount of antigen placed in each well
Show Answer
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Correct Answer: proportional to the amount of antigen placed in each well
2. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
Options
A. The ELISA would not develop when the substrate was added
B. The ELISA would develop normally
C. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
D. None of the above
Show Answer
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Correct Answer: All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
3. Immunoaffinity chromatography can be used in biochemical applications to
Options
A. break down antibody structure
B. purify protein antigen
C. break down antigen and analyze quantitatively
D. none of the above
Show Answer
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Correct Answer: purify protein antigen
4. Which of the following is an immunoelectrophoresis technique?
Options
A. Two-dimensional immunoelectrophoresis
B. Counterimmunoelectrophoresis
C. Coimmunoelectrophoresis
D. Both (a) and (b)
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Correct Answer: Both (a) and (b)
5. An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
Options
A. there was more than one antigen
B. the antibody was impure
C. the temperature was too high
D. there was one antibody and one antigen
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Correct Answer: there was more than one antigen
6. In Counter immunoelectrophoresis
Options
A. electrophoresis will drive the antibody and antigen parallel to each other
B. electrophoresis will drive the antibody and antigen toward each other
C. the antibody will migrate towards anode
D. the antibody will migrate towards cathode
Show Answer
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Correct Answer: electrophoresis will drive the antibody and antigen toward each other
7. DNA- footprinting is a suitable technique for identifying which of the following?
Options
A. Particular mRNA in mixture
B. Particular t-RNA in mixture
C. Introns within DNA
D. Protein binding site within DNA
Show Answer
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Correct Answer: Protein binding site within DNA
8. Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis
Options
A. prior to reaction with antibody
B. prior to reaction with antigen
C. after reaction with antibody
D. after reaction with antigen
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Correct Answer: prior to reaction with antibody
9. The movement of molecules across a membrane by passive transport does not require
Options
A. an input of metabolic energy
B. internal energy
C. vibrational energy
D. none of these
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Correct Answer: an input of metabolic energy
10. Which of the following is correct?
Options
A. Membranes form boundaries around the cell and distinct subcellular components
B. Membranes act as selectively permeable barrier
C. Membranes contain varying amount of lipid and protein and some contain small amount of carbohydrates
D. All of the above
Show Answer
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Correct Answer: All of the above
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Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
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Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Immunological Techniques
Lipid
Membrane Structure and Functions
Minerals
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NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
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