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- Question
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?
Options- A. Open circular, super coiled, linear plasmid DNA
- B. Linear plasmid, open circular, super coiled plasmid
- C. Open circular, super coiled, linear plasmid DNA
- D. Open circular, linear plasmid, super coiled plasmid
- Correct Answer
- Linear plasmid, open circular, super coiled plasmid
- 1. What is the ELISA test intended to measure?
Options- A. Antibody to HIV only
- B. Antigen to HIV only
- C. Presence of free, circulating virus in the patient
- D. Antibodies directed against HLA molecules Discuss
- 2. RAST test (radio allergosorbent test) is often used to detect
Options- A. antibodies associated with allergies (IgE)
- B. antigen associated with allergies (IgE)
- C. bacteriophages
- D. None of the above Discuss
- 3. What would happen if serum is omitted from the ELISA, but all other steps remain same?
Options- A. Anti-human Ig-conjugate would not bind and be washed away
- B. The OD values would be nearly the same as the assay control
- C. Both (a) and (b)
- D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Discuss
- 4. What is approximatively the half life of 32-Phosphorus?
Options- A. 14 days
- B. 28 days
- C. 42 days
- D. 30 days Discuss
- 5. Which of the following is a positively charged matrix for ion exchange chromatography?
Options- A. CM cellulose
- B. DEAE cellulose
- C. Phosphocellulose
- D. None of these Discuss
- 6. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
Options- A. 2000 kb and 20kb DNA
- B. 1000 kb and l0kb DNA
- C. 20 kb and 2000 kb DNA
- D. 10 kb and 1000 kb DNA Discuss
- 7. Which of the following radioactive element is generally involved in RIA?
Options- A. Tritium
- B. Carbon-14
- C. Iodine-125
- D. All of these Discuss
- 8. Radial Immunodiffusion is similar to
Options- A. double-diffusion
- B. gel diffusion
- C. Ouchterloney technique
- D. all of these Discuss
- 9. The IPTG is a gratuitous inducers of (?-galactosidase, and x-gal is a chromogenic substrate. When ?-complementation occurs which colour is produced?
Options- A. Blue
- B. White
- C. Brown
- D. No colour is produced Discuss
- 10. Which of the following is an immunodiffusion test?
Options- A. Double-diffusion
- B. Gel diffusion
- C. Ouchterloney technique
- D. All of these Discuss
Immunological Techniques problems
Search Results
Correct Answer: Antibody to HIV only
Correct Answer: antibodies associated with allergies (IgE)
Correct Answer: Both (a) and (b)
Correct Answer: 14 days
Correct Answer: DEAE cellulose
Correct Answer: 20 kb and 2000 kb DNA
Correct Answer: All of these
Correct Answer: double-diffusion
Correct Answer: Blue
Correct Answer: All of these
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