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  • Question
  • Which of the following is a positively charged matrix for ion exchange chromatography?


  • Options
  • A. CM cellulose
  • B. DEAE cellulose
  • C. Phosphocellulose
  • D. None of these

  • Correct Answer
  • DEAE cellulose 


  • Immunological Techniques problems


    Search Results


    • 1. Rocket Immunodiffusion is also known as

    • Options
    • A. gel diffusion
    • B. electroimmunodiffusion
    • C. double-diffusion
    • D. none of these
    • Discuss
    • 2. The competitive immunoassay can be used

    • Options
    • A. to detect very small amounts of antigen
    • B. to detect antibody associated with allergies (IgE)
    • C. both (a) and (b)
    • D. commonly to detect trace amounts of drugs.
    • Discuss
    • 3. What radioactive molecule is used to end-label an oligonucleotide?

    • Options
    • A. (alpha)-32P-ATP
    • B. (alpha)-32P-dUTP
    • C. (gamma)-32P-ATP
    • D. Any of these
    • Discuss
    • 4. The humoral immune response relies on the production of soluble proteins called

    • Options
    • A. antibodies
    • B. macromolecules
    • C. lymphocytes
    • D. none of these
    • Discuss
    • 5. Antibodies of the IgG class

    • Options
    • A. consist of four subunits
    • B. are glycoproteins
    • C. are secreted into the bloodstream
    • D. all of the above
    • Discuss
    • 6. What is approximatively the half life of 32-Phosphorus?

    • Options
    • A. 14 days
    • B. 28 days
    • C. 42 days
    • D. 30 days
    • Discuss
    • 7. What would happen if serum is omitted from the ELISA, but all other steps remain same?

    • Options
    • A. Anti-human Ig-conjugate would not bind and be washed away
    • B. The OD values would be nearly the same as the assay control
    • C. Both (a) and (b)
    • D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
    • Discuss
    • 8. RAST test (radio allergosorbent test) is often used to detect

    • Options
    • A. antibodies associated with allergies (IgE)
    • B. antigen associated with allergies (IgE)
    • C. bacteriophages
    • D. None of the above
    • Discuss
    • 9. What is the ELISA test intended to measure?

    • Options
    • A. Antibody to HIV only
    • B. Antigen to HIV only
    • C. Presence of free, circulating virus in the patient
    • D. Antibodies directed against HLA molecules
    • Discuss
    • 10. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

    • Options
    • A. Open circular, super coiled, linear plasmid DNA
    • B. Linear plasmid, open circular, super coiled plasmid
    • C. Open circular, super coiled, linear plasmid DNA
    • D. Open circular, linear plasmid, super coiled plasmid
    • Discuss


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