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Biochemistry
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Immunological Techniques
See What Others Are Saying!
Question
What would happen if serum is omitted from the ELISA, but all other steps remain same?
Options
A. Anti-human Ig-conjugate would not bind and be washed away
B. The OD values would be nearly the same as the assay control
C. Both (a) and (b)
D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Correct Answer
Both (a) and (b)
More questions
1. Gluconeogenesis is the
Options
A. formation of glycogen
B. breakdown of glucose to pyruvate
C. breakdown of glycogen to glucose
D. synthesis of glucose from non-carbohydrate precursors
Show Answer
Scratch Pad
Discuss
Correct Answer: synthesis of glucose from non-carbohydrate precursors
2. Which of the following is carried out when cAMP functions as a second messenger?
Options
A. Acts second in importance to AMP
B. Activates all cytosolic protein kinases
C. Activates the cAMP-dependent protein kinase
D. Acts outside the cell to influence cellular processes
Show Answer
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Discuss
Correct Answer: Activates the cAMP-dependent protein kinase
3. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
Options
A. in both cases molecules migrate to the anode
B. both techniques rely on a constant charge to mass ratio
C. both techniques utilize the sieving properties of gels
D. all of the above
Show Answer
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Discuss
Correct Answer: all of the above
4. What process in cellular respiration is essentially the reverse of carbon fixation in photosynthesis?
Options
A. Glycolysis
B. Citric acid cycle
C. Oxidative phosphorylation
D. Alcohol fermentation
Show Answer
Scratch Pad
Discuss
Correct Answer: Citric acid cycle
5. The electrons that are released by the splitting of water during photosynthesis ultimately end up in
Options
A. ATP
B. O
2
C. NADPH
D. rubisco
Show Answer
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Discuss
Correct Answer: NADPH
6. Rubisco (RuBP Carboxylase-oxygenase enzyme), glyceraldehyde 3-phosphate, and NADPH all play a role in
Options
A. the dark reactions of photosynthesis
B. the breakdown of glucose into CO
2
C. cellular respiration when O
2
is present
D. alcohol fermentation
Show Answer
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Discuss
Correct Answer: the dark reactions of photosynthesis
7. Why does the glycolytic pathway continue in the direction of glucose catabolism?
Options
A. There are essentially three irreversible reactions that act as the driving force for the pathway
B. High levels of ATP keep the pathway going in a forward direction
C. The enzymes of glycolysis only function in one direction
D. Glycolysis occurs in either direction
Show Answer
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Discuss
Correct Answer: There are essentially three irreversible reactions that act as the driving force for the pathway
8. A eukaryotic cell that can carry out only fermentation instead of the complete aerobic respiration of glucose
Options
A. produces less CO
2
B. is lacking in O
2
C. has mitochondria present
D. all of these
Show Answer
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Discuss
Correct Answer: all of these
9. Knockout mice are created by
Options
A. mutagenizing a mouse and selecting for mutant offspring
B. creating a chimera by fusing cells from two different cell lines
C. infecting the mouse with a retrovirus
D. transfecting embryonic stem cells with an altered gene sequence
Show Answer
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Discuss
Correct Answer: transfecting embryonic stem cells with an altered gene sequence
10. During the unfolding reaction of a helix, breakage of each hydrogen bond requires about 2kJ/mol. This implies hydrogen bonds are
Options
A. much stronger in proteins than in water
B. not reformed with water
C. slightly weaker in proteins than in water
D. slightly stronger in proteins than in water
Show Answer
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Discuss
Correct Answer: slightly stronger in proteins than in water
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More in Biochemistry:
Allosteric Effects
Amino Acid Metabolism
Anti Bodies
Antigen
ATP Synthesis and Fatty Acid Oxidation
Carbohydrate
Cell Signalling and Transduction
Cell Structure and Compartments
Chromatography
Disease Associated with Immune System
DNA Structure and Replication
Enzymes
FT IR Spectroscopy
Gas Chromatography
Gel Electrophoresis
Genetic Code and Regulation
Genetic Regulation Prokaryotes
Glycolysis
HPLC
Immune Response
Immune System
Immunological Techniques
Lipid
Membrane Structure and Functions
Minerals
Nitrogen Metabolism
NMR Spectroscopy
Nucleic Acids
Oxidative Phosphorylation
Photosynthesis and Respiration
Polymerase Chain Reaction
Protein and Nucleic Acid Interactions
Protein Purification
Protein Stability
Protein Structure
Protein Synthesis
Recombinant DNA Technology
RNA Structure
Spectroscopy
Structure and Properties of Amino Acids
Structure and Properties of Peptides
TCA Cycle
Thermodynamics and Free Energy
Transcription and Regulation
UV Luminance Spectroscopy
Vitamins and Coenzymes
Water, pH and Macromolecules