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Home Biochemistry Immunological Techniques See What Others Are Saying!
  • Question
  • What would happen if serum is omitted from the ELISA, but all other steps remain same?


  • Options
  • A. Anti-human Ig-conjugate would not bind and be washed away
  • B. The OD values would be nearly the same as the assay control
  • C. Both (a) and (b)
  • D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

  • Correct Answer
  • Both (a) and (b) 


  • More questions

    • 1. Gluconeogenesis is the

    • Options
    • A. formation of glycogen
    • B. breakdown of glucose to pyruvate
    • C. breakdown of glycogen to glucose
    • D. synthesis of glucose from non-carbohydrate precursors
    • Discuss
    • 2. Which of the following is carried out when cAMP functions as a second messenger?

    • Options
    • A. Acts second in importance to AMP
    • B. Activates all cytosolic protein kinases
    • C. Activates the cAMP-dependent protein kinase
    • D. Acts outside the cell to influence cellular processes
    • Discuss
    • 3. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

    • Options
    • A. in both cases molecules migrate to the anode
    • B. both techniques rely on a constant charge to mass ratio
    • C. both techniques utilize the sieving properties of gels
    • D. all of the above
    • Discuss
    • 4. What process in cellular respiration is essentially the reverse of carbon fixation in photosynthesis?

    • Options
    • A. Glycolysis
    • B. Citric acid cycle
    • C. Oxidative phosphorylation
    • D. Alcohol fermentation
    • Discuss
    • 5. The electrons that are released by the splitting of water during photosynthesis ultimately end up in

    • Options
    • A. ATP
    • B. O2
    • C. NADPH
    • D. rubisco
    • Discuss
    • 6. Rubisco (RuBP Carboxylase-oxygenase enzyme), glyceraldehyde 3-phosphate, and NADPH all play a role in

    • Options
    • A. the dark reactions of photosynthesis
    • B. the breakdown of glucose into CO2
    • C. cellular respiration when O2 is present
    • D. alcohol fermentation
    • Discuss
    • 7. Why does the glycolytic pathway continue in the direction of glucose catabolism?

    • Options
    • A. There are essentially three irreversible reactions that act as the driving force for the pathway
    • B. High levels of ATP keep the pathway going in a forward direction
    • C. The enzymes of glycolysis only function in one direction
    • D. Glycolysis occurs in either direction
    • Discuss
    • 8. A eukaryotic cell that can carry out only fermentation instead of the complete aerobic respiration of glucose

    • Options
    • A. produces less CO2
    • B. is lacking in O2
    • C. has mitochondria present
    • D. all of these
    • Discuss
    • 9. Knockout mice are created by

    • Options
    • A. mutagenizing a mouse and selecting for mutant offspring
    • B. creating a chimera by fusing cells from two different cell lines
    • C. infecting the mouse with a retrovirus
    • D. transfecting embryonic stem cells with an altered gene sequence
    • Discuss
    • 10. During the unfolding reaction of a helix, breakage of each hydrogen bond requires about 2kJ/mol. This implies hydrogen bonds are

    • Options
    • A. much stronger in proteins than in water
    • B. not reformed with water
    • C. slightly weaker in proteins than in water
    • D. slightly stronger in proteins than in water
    • Discuss


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