Discussion
Home ‣ Biochemistry ‣ Allosteric Effects Comments
- Question
The Hill coefficient (nH) for myoglobin and hemoglobin are respectively
Options- A. 2.8 and 1.0
- B. 1.0 and 2.8
- C. 1.2 and 4.5
- D. 4.5 and 1.2
- Correct Answer
- 1.0 and 2.8
- 1. The best way to determine the location of protein in the purification scheme is to measure the
Options- A. rate of ATP synthesis
- B. changes in the refractive index
- C. UV absorption
- D. mass spectroscopy of the protein Discuss
- 2. Gel-filtration chromatography separates on the basis of
Options- A. size and shape using porous beads packed in a column
- B. size using porous beads packed in a column
- C. shape using porous beads packed in a column
- D. none of the above Discuss
- 3. Proteins separation can be carried out on the basis of
Options- A. net charge
- B. solubility in salt solutions
- C. size or mass
- D. all of these Discuss
- 4. During successful purification scheme, this may be expected that the
Options- A. specific activity increases
- B. specific activity decreases
- C. number of proteins in the sample decreases
- D. both (a) and (c) Discuss
- 5. A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
Options- A. 1,000 units/mg
- B. 10,000 units/mg.
- C. 100,000 units/mg
- D. 1,000,000 units/mg Discuss
- 6. An allosteric activator
Options- A. increases the binding affinity
- B. decreases the binding affinity
- C. stabilizes the R state of the protein
- D. both (a) and (c) Discuss
- 7. The conformational changes from the T to the R state is initiated by
Options- A. binding of oxygen to the heme
- B. movement of the proximal histidine towards the heme
- C. movement of the F-helix, which contains the proximal His
- D. reorganization of protein-protein contacts between the individual subunits Discuss
- 8. When protein binds two ligands in a non-cooperative manner, then the x-intercept of the Scatchard Plot is
Options- A. 1
- B. 2
- C. not defined
- D. none of the above Discuss
- 9. The specificity of a ligand binding site on a protein is based on
Options- A. the absence of competing ligands
- B. the amino acid residues lining the binding site
- C. the presence of hydrating water molecules
- D. the opposite chirality of the binding ligand Discuss
- 10. A protein that binds two ligands in a non-cooperative manner will show
Options- A. a sigmodial binding curve
- B. a hyperbolic binding curve
- C. a linear Scatchard Plot
- D. both (b) and (c) Discuss
Allosteric Effects problems
Search Results
Correct Answer: rate of ATP synthesis
Correct Answer: size and shape using porous beads packed in a column
Correct Answer: all of these
Correct Answer: both (a) and (c)
Correct Answer: 100,000 units/mg
Correct Answer: both (a) and (c)
Correct Answer: binding of oxygen to the heme
Correct Answer: 2
Correct Answer: the amino acid residues lining the binding site
Correct Answer: both (b) and (c)
Comments
There are no comments.More in Biochemistry:
Programming
Copyright ©CuriousTab. All rights reserved.