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- Question
Gel-filtration chromatography separates on the basis of
Options- A. size and shape using porous beads packed in a column
- B. size using porous beads packed in a column
- C. shape using porous beads packed in a column
- D. none of the above
- Correct Answer
- size and shape using porous beads packed in a column
- 1. Proteins separation can be carried out on the basis of
Options- A. net charge
- B. solubility in salt solutions
- C. size or mass
- D. all of these Discuss
- 2. During successful purification scheme, this may be expected that the
Options- A. specific activity increases
- B. specific activity decreases
- C. number of proteins in the sample decreases
- D. both (a) and (c) Discuss
- 3. A purified protein sample contains 10 ?g of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
Options- A. 1,000 units/mg
- B. 10,000 units/mg.
- C. 100,000 units/mg
- D. 1,000,000 units/mg Discuss
- 4. Affinity chromatography deals with the
Options- A. specific binding of a protein constituents for another molecule
- B. protein - protein interaction
- C. protein - carbohydrate interaction
- D. none of the above Discuss
- 5. Which of the following may be added to stabilize the protein after yeast cells disruption?
Options- A. NaCl
- B. Protease inhibitor
- C. AMP
- D. All of these Discuss
- 6. The best way to determine the location of protein in the purification scheme is to measure the
Options- A. rate of ATP synthesis
- B. changes in the refractive index
- C. UV absorption
- D. mass spectroscopy of the protein Discuss
- 7. The Hill coefficient (nH) for myoglobin and hemoglobin are respectively
Options- A. 2.8 and 1.0
- B. 1.0 and 2.8
- C. 1.2 and 4.5
- D. 4.5 and 1.2 Discuss
- 8. An allosteric activator
Options- A. increases the binding affinity
- B. decreases the binding affinity
- C. stabilizes the R state of the protein
- D. both (a) and (c) Discuss
- 9. The conformational changes from the T to the R state is initiated by
Options- A. binding of oxygen to the heme
- B. movement of the proximal histidine towards the heme
- C. movement of the F-helix, which contains the proximal His
- D. reorganization of protein-protein contacts between the individual subunits Discuss
- 10. When protein binds two ligands in a non-cooperative manner, then the x-intercept of the Scatchard Plot is
Options- A. 1
- B. 2
- C. not defined
- D. none of the above Discuss
Protein Purification problems
Search Results
Correct Answer: all of these
Correct Answer: both (a) and (c)
Correct Answer: 100,000 units/mg
Correct Answer: specific binding of a protein constituents for another molecule
Correct Answer: All of these
Correct Answer: rate of ATP synthesis
Correct Answer: 1.0 and 2.8
Correct Answer: both (a) and (c)
Correct Answer: binding of oxygen to the heme
Correct Answer: 2
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